Pesáková V, Smetana K, Sochor M, Hulejová H, Balík K
Rheumatological Institute Na Slupi 4, Prague 2, 128 50, Czech Republic.
J Mater Sci Mater Med. 2005 Feb;16(2):143-8. doi: 10.1007/s10856-005-5933-7.
Intervertebral cages are used in orthopaedics for stabilization of injured lumbar parts of vertebral columns. Our study provides preliminary results of tests of the biological properties of titanium cages with a variously modified carbon/carbon composite (C/C) core. This core was produced from a C/C composite modified by hydrogel materials based on poly(2-hydroxyethyl methacrylate) (HEMA) enriched with 1% collagen or 35% methylmethacrylate or 30% terc-butylmethacrylamide. We evaluated the adhesion of the cells to the tested material coating using an in vitro study of the metabolic activity and cytokine production of the cells (TNF-alpha, IL-8). We studied the biocompatibility of intervertebral cages coated with different copolymers under in vivo condition and in an implantation experiment in the porcine femurs. Both in vitro and in vivo results revealed favourable biotolerance of the use system. Modification of the composite HEMA with the use of collagen seems to have a more positive effect on the new bone tissue formed around the implanted devices than HEMA copolymerized with methylmethacrylate or terc-butylmethacrylamide.
椎间融合器在骨科用于稳定脊柱受伤的腰椎部分。我们的研究提供了对具有不同改性碳/碳复合材料（C/C）核心的钛制融合器生物学特性测试的初步结果。该核心由基于聚（甲基丙烯酸2-羟乙酯）（HEMA）的水凝胶材料改性的C/C复合材料制成，其中富含1%的胶原蛋白、35%的甲基丙烯酸甲酯或30%的叔丁基甲基丙烯酰胺。我们通过对细胞代谢活性和细胞因子产生（肿瘤坏死因子-α、白细胞介素-8）的体外研究，评估了细胞对测试材料涂层的粘附性。我们在体内条件下以及在猪股骨植入实验中研究了涂覆不同共聚物的椎间融合器的生物相容性。体外和体内结果均显示该使用系统具有良好的生物耐受性。与与甲基丙烯酸甲酯或叔丁基甲基丙烯酰胺共聚的HEMA相比，使用胶原蛋白对复合材料HEMA进行改性似乎对植入装置周围形成的新骨组织具有更积极的影响。
