Alat Ilker, Inan Muharrem, Gurses Iclal, Kekilli Ersoy, Germen Burak, Harma Ahmet, Eskin Ahmet, Aydin Omer Murat
Department of Cardiovascular Surgery, Inonu University Medical Faculty, Turgut Ozal Medical Center, 44069 Malatya, Turkey.
Tex Heart Inst J. 2004;31(4):363-7.
We induced angiogenesis in the tibial medulla and cortex of rabbits by electrical and mechanical stimulation, with the aim of future application to ischemic disease. Sixteen New Zealand rabbits were divided into 4 groups: in Group 1, a K wire was inserted into the medullary channel; in Group 2 a hole was drilled into the tibia; in Group 3, electrical stimulation was applied to the medullary channel; and in Group 4 (the control group), nothing was done. The interventions were applied during a 21-day period, after which all animals were evaluated scintigraphically and histopathologically. All 3 interventional groups were significantly superior to the control group in regard to medullary and cortical vascularity: the P values were 0.021 in all comparisons to control. However, the most fibrotic changes in the medulla occurred in the group that had been treated with electricity (P = 0.008). Slight fibrotic changes occurred in the hole group (P = 0.040), and none occurred in the K-wire group. In sum, all 3 interventions are capable of inducing medullary angiogenesis, but electricity is inferior in regard to fibrotic change. We believe that this present study can establish a baseline for further work that explores clinical applications to problematic ischemic conditions, including delayed sternal wound healing after cardiac surgery.
我们通过电刺激和机械刺激诱导兔胫骨骨髓和皮质的血管生成，目的是未来应用于缺血性疾病。16只新西兰兔被分为4组：第1组，将一根K线插入髓腔；第2组，在胫骨上钻孔；第3组，对髓腔施加电刺激；第4组（对照组）不做任何处理。干预措施在21天内实施，之后对所有动物进行闪烁扫描和组织病理学评估。在骨髓和皮质血管形成方面，所有3个干预组均显著优于对照组：与对照组的所有比较中P值均为0.021。然而，接受电刺激治疗的组骨髓中纤维化变化最为明显（P = 0.008）。钻孔组出现轻微纤维化变化（P = 0.040），K线组未出现纤维化变化。总之，所有3种干预措施都能够诱导骨髓血管生成，但电刺激在纤维化变化方面较差。我们认为，本研究可为进一步探索临床应用于有问题的缺血性疾病（包括心脏手术后胸骨伤口愈合延迟）的工作奠定基础。
