Morra Marco, Cassinelli Clara, Meda Laura, Fini Milena, Giavaresi Gianluca, Giardino Roberto
Nobil Bio Ricerche, Str. S. Rocco 36, 14018 Villafranca d'Asti, Italy.
Int J Oral Maxillofac Implants. 2005 Jan-Feb;20(1):23-30.
The aim of this study was to evaluate the surface chemistry and the microhardness at the implant-bone interface using a recently developed collagen-coated titanium implant in a short-term rabbit model.
Surface chemistry was evaluated by x-ray photoelectron spectroscopy (XPS), while in vivo studies involved 4-week implants mid-diaphysis in the lateral femurs of adult male rabbits. After conventional embedding and evaluation of histologic sections, the resinembedded blocks containing the implanted screws were used to measure bone hardness by means of an indentation test.
Decomposition of the C1s peak obtained by XPS analysis confirmed that surface-immobilized collagen retained all the molecular features of the control, nonimmobilized reference. As to microhardness measurement, newly formed bone at the collagen-coated-implant/bone interface was significantly harder than bone at the interface of the uncoated control implant and bone.
These results suggested that collagen coating significantly improves bone maturation and mineralization at the interface in comparison with uncoated commercially pure titanium. Surface modification of titanium implants by collagen coating has recently been discussed as a promising approach to the biochemical modification of implant surfaces. The present results support previous histologic findings and demonstrated that the biomolecular layer linked over the titanium implant can increase the bone healing rate, at least in this animal model.
The present microhardness measurement at the bone-implant interface showed that collagen coating can significantly improve bone maturation and mineralization at the interface in comparison with uncoated commercially pure titanium, confirming and substantiating previous findings by histomorphometric measurements from the same model.
本研究的目的是在短期兔模型中，使用最近开发的胶原涂层钛植入物，评估植入物与骨界面的表面化学性质和显微硬度。
通过X射线光电子能谱（XPS）评估表面化学性质，而体内研究涉及在成年雄性兔的股骨外侧骨干中部植入4周的植入物。在对组织切片进行常规包埋和评估后，使用含有植入螺钉的树脂包埋块通过压痕试验测量骨硬度。
通过XPS分析获得的C1s峰分解证实，表面固定的胶原保留了对照非固定参考物的所有分子特征。至于显微硬度测量，胶原涂层植入物/骨界面处新形成的骨明显比未涂层对照植入物与骨界面处的骨更硬。
这些结果表明，与未涂层的商业纯钛相比，胶原涂层显著改善了界面处的骨成熟和矿化。最近，通过胶原涂层对钛植入物进行表面改性已被视为一种有前景的植入物表面生化改性方法。本研究结果支持了先前的组织学研究结果，并表明连接在钛植入物上的生物分子层可以提高骨愈合率，至少在该动物模型中如此。
目前在骨-植入物界面进行的显微硬度测量表明，与未涂层的商业纯钛相比，胶原涂层可显著改善界面处的骨成熟和矿化，证实并充实了同一模型组织形态计量学测量的先前结果。
