Fedorova N E, Madzhidova M G, Vorontsova O N, Degtiarev D N, Pustowoit B, Asadi Mobarkhan S M, Samatova M M, Kushch A A, Volodin N N
Vopr Virusol. 2005 Jan-Feb;50(1):9-14.
31 prematures with signs of the cytomegalovirus infection (CMV) were examined. The blood and urine samples were tested for direct viral markers, i.e. for infectious CMV by the rapid culture method (RCM) and for viral DNA by quantitative PCR. Besides, the parameters of the specific immune response were studied in the babies. CMV was detected by RCM and/or PCR in 25 of the 31 examined babies during their 1st life week. The highest content of CMV within the investigated samples, i.e. 100 antigen-containing cells per 2.5 x 10(5) culture cells and above 2000 copies/ml of viral DNA was detected in 8 (32%) children. The quantity of viral DNA did not exceed 1000 copies/ml and one to three of stained cells was detected by PCR in 13 (42%) children. A study of anti-CMV in sera revealed high-titer of AT IgG in all 30 children. High avidity of anti-CMV-IgG was demonstrated to correlate with a low viral load and a low CMV infection activity in the newborns. According to the results, at least 3 laboratory diagnosis tools should be used in the diagnosis, they are PCR, RCM and determination of the anti-CMV avidity.
对31例有巨细胞病毒感染（CMV）迹象的早产儿进行了检查。对血液和尿液样本进行了直接病毒标志物检测，即通过快速培养法（RCM）检测感染性CMV，通过定量PCR检测病毒DNA。此外，还研究了这些婴儿的特异性免疫反应参数。在31例受检婴儿出生后的第一周内，通过RCM和/或PCR在其中25例中检测到了CMV。在8名（32%）儿童的检测样本中检测到了所研究样本中最高含量的CMV，即每2.5×10⁵个培养细胞中有100个含抗原细胞且病毒DNA含量高于2000拷贝/ml。在13名（42%）儿童中，病毒DNA含量不超过1000拷贝/ml，通过PCR检测到一至三个染色细胞。对血清中抗CMV的研究显示，所有30名儿童的抗CMV IgG均为高滴度。结果表明，抗CMV IgG的高亲和力与新生儿的低病毒载量和低CMV感染活性相关。根据结果，在诊断中至少应使用3种实验室诊断工具，即PCR、RCM和抗CMV亲和力测定。
