Karganova G G, Pripuzova N S, Tereshkina N V, Gmyl' L V, Dzhivanian T I, Rumiantsev A A, Lashkevich V A
Vopr Virusol. 2005 Jan-Feb;50(1):27-31.
The residual neurovirulence of the previously obtained chimeric virus Tp21/DEN4, containing the RNA region encoding the pre M and E structural enzymes, strain Tp21, virus Langat, and the remaining part of the genome from the Denge 4 virus, was studied in experiments with monkeys Cercopithecus aethiops involving the intracerebral administration of the virus. The tick-borne encephalitis virus, strain Absettarov, was used as positive control. A comparative analysis of the experimental and published data showed the chimeric virus to be less virulent by its degree of morphological affection in the CNS zones, its spread into the CNS and by a percentage share of animals with viremia versus the Tp21 parent strain and Elantsev strain of the Langat virus.
在对猕猴（非洲绿猴）进行的涉及脑内接种病毒的实验中，研究了先前获得的嵌合病毒Tp21/DEN4的残余神经毒力。该嵌合病毒包含编码前M和E结构酶的RNA区域、Tp21毒株、Langat病毒，以及登革4型病毒基因组的其余部分。蜱传脑炎病毒Absettarov毒株用作阳性对照。通过对实验数据和已发表数据的比较分析表明，相较于Tp21亲代毒株和Langat病毒的Elantsev毒株，嵌合病毒在中枢神经系统区域的形态学影响程度、在中枢神经系统中的传播以及病毒血症动物的百分比方面，毒力较低。
