Hamrin K, Henriksson J
Department of Physiology and Pharmacology, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
Life Sci. 2005 Apr 1;76(20):2329-38. doi: 10.1016/j.lfs.2004.11.008. Epub 2005 Jan 26.
The aim of this study was to investigate the local effect of the insulin-mimetic agent vanadate on glucose metabolism in human skeletal muscle in vivo. Interstitial concentrations of glucose and lactate were determined by microdialysis at a low flow rate in the quadriceps femoris muscle of 18 men. In the same leg two microdialysis catheters were inserted. In one catheter, the perfusion medium was supplemented with sodium metavanadate (10-100 mM) after a basal period, the other catheter served as control. In the catheter perfused with metavanadate, the interstitial glucose concentration was decreased by 13-50% compared to the control catheter (p<0.05). The lactate concentration was higher in the 50 mM and 100 mM metavanadate catheters compared to control (39-89%, p<0.05). There was no difference between control and metavanadate catheters in urea concentrations. Five of the subjects were insulin-resistant and for them the results were similar, although the effect was somewhat smaller. The decreased interstitial glucose concentration, and the increased lactate concentration, in the vicinity of the microdialysis catheter most likely reflects an increased cellular glucose uptake. The present study thus indicates that vanadate mimics the effect of insulin in human skeletal muscle in vivo.
本研究的目的是调查胰岛素模拟剂钒酸盐对人体骨骼肌葡萄糖代谢的局部体内效应。通过微透析法，在18名男性股四头肌处以低流速测定葡萄糖和乳酸的间质浓度。在同一条腿上插入两根微透析导管。一根导管在基础期后向灌注介质中添加偏钒酸钠（10 - 100 mM），另一根导管作为对照。与对照导管相比，在灌注偏钒酸钠的导管中，间质葡萄糖浓度降低了13 - 50%（p<0.05）。与对照相比，50 mM和100 mM偏钒酸钠导管中的乳酸浓度更高（39 - 89%，p<0.05）。对照导管和偏钒酸钠导管中的尿素浓度没有差异。其中5名受试者存在胰岛素抵抗，对他们而言结果相似，尽管效应稍小。微透析导管附近间质葡萄糖浓度降低和乳酸浓度升高，很可能反映了细胞对葡萄糖摄取增加。因此，本研究表明钒酸盐在体内模拟了胰岛素对人体骨骼肌的作用。
