Division of Translational Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA.
Burleson Research Technologies, Inc, Morrisville, North Carolina, USA.
J Appl Toxicol. 2023 Nov;43(11):1686-1701. doi: 10.1002/jat.4508. Epub 2023 Jul 5.
Sodium metavanadate (NaVO ) is a pentavalent vanadium compound used in the metal industry and dietary supplements; human exposure occurs through inhalation of fumes and dust and ingestion of NaVO -containing products. The objective of this study was to assess the potential immunotoxicity of NaVO . Female B6C3F1/N mice were exposed to 0-500 ppm NaVO in drinking water for 28 days and evaluated for effects on immune cell populations and innate, cellular-mediated, and humoral-mediated immunity. There was a decreasing trend in body weight (BW) and BW gain in NaVO exposed mice, with a decrease (p ≤ 0.05) in BW gain at ≥250 ppm, relative to control. Conversely, increasing trends in spleen weights and an increase (p ≤ 0.05) in the spleen:BW ratio at ≥250 ppm NaVO were observed. NaVO exposure altered antibody production against sheep red blood cells (SRBC). Antibody forming cells (AFC)/10 spleen cells exhibited a decreasing trend, with a decrease (p ≤ 0.05) at 500 ppm NaVO , concurrent with an increase in percent B cells. NaVO had no effect on the serum anti-SRBC IgM antibody titers or anti-keyhole limpet hemocyanin antibody production. Exposure to NaVO decreased the percentage of natural killer cells at all dose levels (p ≤ 0.05), with no effect on the lytic activity. NaVO altered T-cell populations at 500 ppm but had no effect on T-cell proliferative responses or the lytic activity of cytotoxic T cells. Collectively, these data indicate that NaVO exposure can adversely affect the immune system by inducing alterations in humoral-mediated immunity, specifically the AFC response, with no effect on cell-mediated or innate immunity.
偏钒酸钠（NaVO ）是一种五价钒化合物，用于金属工业和膳食补充剂；人类通过吸入烟雾和粉尘以及摄入含 NaVO 的产品而接触到它。本研究的目的是评估 NaVO 的潜在免疫毒性。雌性 B6C3F1/N 小鼠通过饮用水暴露于 0-500ppm NaVO ，为期 28 天，并评估对免疫细胞群以及先天、细胞介导和体液介导免疫的影响。暴露于 NaVO 的小鼠的体重（BW）和体重增加呈下降趋势，与对照组相比，在≥250ppm 时体重增加减少（p≤0.05）。相反，观察到脾脏重量呈增加趋势，并且在≥250ppm NaVO 时脾脏：BW 比值增加（p≤0.05）。NaVO 暴露改变了针对绵羊红细胞（SRBC）的抗体产生。抗体形成细胞（AFC）/10 个脾脏细胞呈下降趋势，在 500ppm NaVO 时下降（p≤0.05），同时 B 细胞百分比增加。NaVO 对血清抗 SRBC IgM 抗体滴度或抗血蓝蛋白抗体的产生没有影响。暴露于 NaVO 降低了所有剂量水平的自然杀伤细胞的百分比（p≤0.05），对裂解活性没有影响。NaVO 改变了 500ppm 时的 T 细胞群，但对 T 细胞增殖反应或细胞毒性 T 细胞的裂解活性没有影响。总的来说，这些数据表明，NaVO 暴露通过诱导体液介导免疫的改变，特别是 AFC 反应，对细胞介导或先天免疫没有影响，从而可能对免疫系统产生不利影响。
