Flores-Cotera Luis B, García-Salas Sergio
Department of Biotechnology and Bioengineering, Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN No. 2508, México, D.F. 07360, Mexico.
J Biotechnol. 2005 Apr 6;116(4):387-96. doi: 10.1016/j.jbiotec.2004.12.011.
A concentric draft tube jet loop bioreactor (10.5 m3) was used to study the influence of aerated liquid height (above the draft tube) on the amount of surfactant addition allowable without foaming. Sodium lauryl sulfate (SLS) and defatted soybean flour in tap water were used as model artificial media. The amount of surfactant required to develop foaming and the maximum gas holdup achieved prior to foaming were notably influenced by aerated liquid height. Decreasing the aerated liquid height from 1.50 to 0.05 m increased the amount of SLS allowed without foaming from 2.2 to 12.1 g, the gas holdup in the riser from 0.18 to 0.31 and the gas holdup in the downcomer from 0.12 to 0.25. Similar behavior was observed for soybean flour. Decreasing the aerated liquid height from 1.45 to 0.05 m increased the amount of soybean flour allowed without foaming from 822 to 3200 g, the gas holdup in the riser from 0.17 to 0.26 and the gas holdup in the downcomer from 0.10 to 0.19. Data from a representative continuous yeast culture are reported to show that operation at low aerated liquid heights (0.5 m) can also be used to produce a culture broth with large gas holdup and oxygen transfer but without foaming.
使用一个同心导流筒喷射环流生物反应器（10.5立方米）来研究曝气液体高度（导流筒上方）对不产生泡沫情况下允许添加的表面活性剂数量的影响。将自来水中的十二烷基硫酸钠（SLS）和脱脂大豆粉用作模拟人工培养基。产生泡沫所需的表面活性剂数量以及发泡前达到的最大气体滞留率显著受曝气液体高度的影响。将曝气液体高度从1.50米降低至0.05米，可使不产生泡沫情况下允许的SLS量从2.2克增加至12.1克，提升管中的气体滞留率从0.18增加至0.31，降液管中的气体滞留率从0.12增加至0.25。对于大豆粉也观察到了类似行为。将曝气液体高度从1.45米降低至0.05米，可使不产生泡沫情况下允许的大豆粉量从822克增加至3200克，提升管中的气体滞留率从0.17增加至0.26，降液管中的气体滞留率从0.10增加至0.19。报告了来自典型连续酵母培养的数据，以表明在低曝气液体高度（0.5米）下运行也可用于生产具有高气体滞留率和氧传递但不产生泡沫的培养液。
