通过与LA-N-1神经母细胞瘤细胞的交叉反应性进行抗MAG IgM抗体的免疫检测。

Immunodetection of anti-MAG IgM antibody by cross-reactivity to LA-N-1 neuroblastoma cells.

作者信息

Isoardo Gianluca, Deaglio Silvia, Cocito Dario, Migliaretti Giuseppe, Ferrero Enza, Cavallo Franco, Durelli Luca, Malavasi Fabio

机构信息

SOC Neurologia, Ospedale Civile di Asti, 14100 Asti, Italy.

出版信息

J Neuroimmunol. 2005 Apr;161(1-2):78-86. doi: 10.1016/j.jneuroim.2004.12.005.

DOI:10.1016/j.jneuroim.2004.12.005

PMID:15748946

Abstract

Demyelinating polyneuropathiy associated with IgM paraproteinemia and high titers of anti-MAG IgM antibodies (MAG-PN) is considered different from chronic inflammatory demyelinating polyneuropathy, particularly because of the poorer response to treatment of MAG-PN patients. Therefore, anti-MAG anitbodies may have relevant prognostic value. Available anti-MAG antibody assays require central nervous system myelin proteins from autopsied human brains. This study investigated the feasibility of detecting anti-MAG antibody by immunofluorescence and flow cytometry using a panel of human neuroblastoma cell lines as targets. We report here on the evaluation of the LA-N-1 cell line as an appropriate substrate for the detection of anti-MAG antibody by indirect immunoflourescence.

摘要

与IgM副蛋白血症及高滴度抗髓鞘相关糖蛋白（MAG）IgM抗体相关的脱髓鞘性多发性神经病（MAG-PN）被认为与慢性炎症性脱髓鞘性多发性神经病不同，尤其是因为MAG-PN患者对治疗的反应较差。因此，抗MAG抗体可能具有相关的预后价值。现有的抗MAG抗体检测需要来自尸检人脑的中枢神经系统髓磷脂蛋白。本研究调查了以一组人神经母细胞瘤细胞系为靶点，通过免疫荧光和流式细胞术检测抗MAG抗体的可行性。我们在此报告关于LA-N-1细胞系作为通过间接免疫荧光检测抗MAG抗体的合适底物的评估。