Groneberg David A, Serowka Frauke, Peckenschneider Nora, Artuc Metin, Grützkau Andreas, Fischer Axel, Henz Beate M, Welker Pia
Allergy-Centre-Charité, Charité School of Medicine, Free and Humboldt University, Augustenburger Platz 1 OR-1, D-13353 Berlin, Germany.
J Neuroimmunol. 2005 Apr;161(1-2):87-92. doi: 10.1016/j.jneuroim.2004.12.019.
The gene expression and regulation of nerve growth factor (NGF) in atopic dermatitis (AD) and the human mast cell line (HMC)-1 was investigated at the molecular level. NGF-stimulation of HMC-1 cells resulted in increases in tryptase activity and histamine contents, paralleled by an increase of tryptase and histamine at the transcriptional level. Also, an increased expression of NGF was found in AD lesions, in association with increased systemic NGF plasma levels. Further cutaneous sources for increased NGF levels were keratinocytes and fibroblasts. These findings demonstrate an increased expression of NGF in AD and effects on tryptase and histamine. Mast cells may be major mediators of neurotrophin effects in AD.
在分子水平上研究了特应性皮炎（AD）和人肥大细胞系（HMC）-1中神经生长因子（NGF）的基因表达及调控。NGF刺激HMC-1细胞导致类胰蛋白酶活性和组胺含量增加，同时类胰蛋白酶和组胺在转录水平也增加。此外，在AD皮损中发现NGF表达增加，同时全身血浆NGF水平也升高。NGF水平升高的其他皮肤来源是角质形成细胞和成纤维细胞。这些发现表明AD中NGF表达增加及其对类胰蛋白酶和组胺的影响。肥大细胞可能是AD中神经营养因子作用的主要介质。
