外源性血小板活化因子处理精子对宫腔内人工授精结局的影响。
Effect of sperm treatment with exogenous platelet-activating factor on the outcome of intrauterine insemination.
作者信息
Grigoriou Odysseas, Makrakis Evangelos, Konidaris Socrates, Hassiakos Dimitrios, Papadias Konstantinos, Baka Stavroula, Creatsas George
机构信息
Assisted Reproduction Unit, 2nd Department of Obstetrics and Gynecology, University of Athens, Aretaieion Hospital, Athens, Greece.
出版信息
Fertil Steril. 2005 Mar;83(3):618-21. doi: 10.1016/j.fertnstert.2004.07.980.
OBJECTIVE
To evaluate the effect of sperm treatment with exogenous platelet-activating factor (PAF) on IUI clinical pregnancy rate.
DESIGN
Prospective randomized study.
SETTING
Assisted Reproduction Unit, 2nd Department of Obstetrics and Gynecology, University of Athens, Aretaieion Hospital, Athens, Greece.
SUBJECT(S): Fifty-two couples with unexplained infertility, candidates for IUI.
INTERVENTION(S): Sperm treatment with an exogenous mixture of PAF (final concentration, 10(-7) mol/L) in sperm-washing medium, direct swim-up technique of sperm preparation, a maximum of six IUI cycles per couple with or without PAF treatment.
MAIN OUTCOME MEASURE(S): Clinical pregnancy rate (pregnancies confirmed by ultrasonography per hundred cycles).
RESULT(S): The overall clinical pregnancy rate after a maximum of six IUI cycles was significantly higher when sperm was treated with PAF compared with the rate after the direct swim-up technique (23.07% vs. 7.92%).
CONCLUSION(S): Treatment of sperm with exogenous PAF might improve the clinical outcome of IUI in cases of unexplained infertility.
目的
评估用外源性血小板激活因子（PAF）处理精子对宫腔内人工授精（IUI）临床妊娠率的影响。
设计
前瞻性随机研究。
地点
希腊雅典阿雷泰翁医院雅典大学第二妇产科辅助生殖科。
研究对象
52对不明原因不孕且适合进行IUI的夫妇。
干预措施
在精子洗涤培养基中用外源性PAF混合物（终浓度为10⁻⁷mol/L）处理精子，采用直接上游法制备精子，每对夫妇最多进行6个IUI周期，分为接受或不接受PAF处理两组。
主要观察指标
临床妊娠率（每100个周期经超声检查确诊的妊娠数）。
结果
最多进行6个IUI周期后，用PAF处理精子时的总体临床妊娠率显著高于直接上游法处理精子时的妊娠率（23.07%对7.92%）。
结论
在不明原因不孕的情况下，用外源性PAF处理精子可能会改善IUI的临床结局。
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