Van Dyck E, Smet H, Van Damme L, Laga M
STD/HIV Research and Intervention Unit, Department of Microbiology, Institute of Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium.
J Clin Microbiol. 2001 Jun;39(6):2280-2. doi: 10.1128/JCM.39.6.2280-2282.2001.
The AMPLICOR PCR was used to detect Neisseria gonorrhoeae in endocervical specimens. A 16S rRNA PCR performed on N. gonorrhoeae-positive samples showed sensitivities of 73.2, 64.3, and 94.4% for samples treated directly with AMPLICOR lysis buffer, samples suspended in 2-sucrose phosphate, and samples suspended in diluted phosphate-buffered saline, respectively.
使用AMPLICOR聚合酶链反应(PCR)检测宫颈标本中的淋病奈瑟菌。对淋病奈瑟菌阳性样本进行的16S核糖体RNA(rRNA)PCR显示,直接用AMPLICOR裂解缓冲液处理的样本、悬浮于2-蔗糖磷酸盐中的样本以及悬浮于稀释磷酸盐缓冲盐水中的样本的敏感度分别为73.2%、64.3%和94.4%。