Zhang Liangfen, Namisato Masako, Matsuoka Masanori
Leprosy Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
Int J Lepr Other Mycobact Dis. 2004 Dec;72(4):468-72. doi: 10.1489/1544-581X(2004)72<468:AMACIT>2.0.CO;2.
A missense mutation at codon 516 in the rpoB gene of Mycobacterium leprae conferring rifampin resistance was confirmed by the correlation between sequencing results and mouse footpad assay. The isolate was obtained from a relapsed lepromatous leprosy patient. This is the first report on the complete concordance between the mutation located at codon 516 in the rpoB gene and the corresponding resistance to rifampin in leprosy. The novel profile of mutation in the rpoB gene will contribute to the comprehensive understanding of rifampin resistant patterns and offer a useful tool for developing simple and rapid drug susceptibility testing approaches, which would promise more effective and successful control of leprosy.
通过测序结果与小鼠足垫试验之间的相关性，证实了麻风分枝杆菌rpoB基因第516密码子处的一个错义突变赋予了对利福平的抗性。该分离株来自一名复发的瘤型麻风患者。这是关于rpoB基因第516密码子处的突变与麻风病中相应的利福平抗性完全一致的首次报道。rpoB基因的新型突变谱将有助于全面了解利福平耐药模式，并为开发简单快速的药敏试验方法提供有用工具，这有望更有效且成功地控制麻风病。
