Honoré N, Perrani E, Telenti A, Grosset J, Cole S T
Unité de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France.
Int J Lepr Other Mycobact Dis. 1993 Dec;61(4):600-4.
The rifampin resistance of Mycobacterium leprae is due to missense mutations in the rpoB gene encoding the beta-subunit of the essential enzyme RNA polymerase. A rapid and very simple method has been developed to detect rifampin resistance in small numbers of M. leprae present in biopsies. It involves polymerase chain reaction amplification of a defined region of the rpoB gene followed by single-strand conformational polymorphism analysis (PCR-SSCP). The reliability of the method has been tested on a sample of known drug-resistant and susceptible isolates of M. leprae.
麻风分枝杆菌对利福平的耐药性是由于编码必需酶RNA聚合酶β亚基的rpoB基因发生错义突变。现已开发出一种快速且非常简单的方法，用于检测活检组织中少量麻风分枝杆菌的利福平耐药性。该方法包括对rpoB基因的特定区域进行聚合酶链反应扩增，随后进行单链构象多态性分析（PCR-SSCP）。已在一组已知的麻风分枝杆菌耐药和敏感菌株样本上测试了该方法的可靠性。
