Sapkota Bishwa Raj, Ranjit Chaman, Macdonald Murdo
Leprosy Mission Nepal, Mycobacterial Research Laboratory, Anandaban Hospital, Kathmandu, Nepal.
Nepal Med Coll J. 2006 Jun;8(2):122-7.
Mutations in the rpoB gene of 40 biopsy isolates of Mycobacterium leprae were analyzed by reverse hybridization-based line probe assay after PCR, and nine distinct single-nucleotide substitutions were found. Among them, a 3-nucleotide substitution was found in two, and 2-nucleotide substitutions were found in seven isolates. This is a new finding of multiple mutations in a single point of the rpoB gene for rifampicin resistance. This investigation demonstrates that the pattern of mutations in the rpoB gene for rifampicin resistance in Nepal involves more variety.
采用基于反向杂交的线性探针分析法，对40株麻风分枝杆菌活检分离株的rpoB基因进行PCR扩增后分析，发现了9种不同的单核苷酸替换。其中，有2株出现3个核苷酸替换，7株出现2个核苷酸替换。这是rpoB基因单个位点出现多重突变导致利福平耐药的新发现。这项研究表明，尼泊尔rpoB基因中利福平耐药的突变模式更为多样。
