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真菌遗传资源中心与基因组挑战。

Fungal genetic resource centres and the genomic challenge.

作者信息

Ryan Matthew J, Smith David

机构信息

Genetic Resources Collection, CABI Bioscience UK Centre, Bakeham Lane, Egham, Surrey TW20 9TY, UK.

出版信息

Mycol Res. 2004 Dec;108(Pt 12):1351-62. doi: 10.1017/s0953756204001650.

Abstract

Fungal research and education has for many years been supported by public service genetic resource centres, whose roles have been to maintain, preserve and supply living cultures to the research community. In the genomic era, genetic resource centres are perhaps more important than ever before. The cultures held, many of which are described and validated by expert biosystematists, are valuable resources for the future. There is a need to supply genomic and proteomic research programmes with fully characterised organisms, as usage of organisms from unreliable sources can prove disastrous, not least in economical terms. However, mycologists often require more than just the organisms, for example, their associated information is vital for bioinformatic applications and some researchers may only require genomic DNA from the organism rather than the organism per se. Genetic resource centres are continually adapting to meet the needs of their users and the wider mycological research community, this associated with OECD international initiatives should ensure they exist to support research for many years to come. This review considers the impact of such initiatives, the current roles of fungal genetic resource centres, the mechanisms used to preserve organisms in a stable manner and the range of resources that are offered for genomic research.

摘要

多年来,真菌研究与教育一直得到公共服务遗传资源中心的支持,这些中心的作用是维护、保存并向研究团体提供活培养物。在基因组时代,遗传资源中心或许比以往任何时候都更为重要。所保存的培养物中有许多已由专业生物分类学家进行了描述和验证,它们是未来的宝贵资源。有必要为基因组学和蛋白质组学研究项目提供特征明确的生物体,因为使用来源不可靠的生物体可能会造成灾难性后果,尤其是在经济方面。然而,真菌学家通常需要的不仅仅是生物体本身,例如,其相关信息对于生物信息学应用至关重要,而且一些研究人员可能只需要生物体的基因组DNA而非生物体本身。遗传资源中心不断调整以满足用户及更广泛真菌学研究团体的需求,这与经合组织的国际倡议相结合,应能确保它们在未来多年继续存在以支持研究工作。本综述探讨了这些倡议的影响、真菌遗传资源中心目前的作用、以稳定方式保存生物体所采用的机制以及为基因组研究提供的资源范围。

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