Przybyło M, Lityńska A, Pocheć E
Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Ingardena 6, 30-060 Kraków, Poland.
Biochimie. 2005 Feb;87(2):133-42. doi: 10.1016/j.biochi.2004.12.003.
In tumour cells, alterations in cellular glycosylation may play a key role in their metastatic behaviour. This study used cell lines having very different behaviour in vivo: HCV29 non-malignant transitional epithelium and T24 bladder transitional cell carcinoma. These differences in behaviour might be due in part to differences in cellular glycosylation patterns. Glycan chain analysis of their glycoproteins was performed with the use of specific lectins. The functional role of carbohydrates was studied by treating these cells with swainsonine, an inhibitor of Golgi alpha-mannosidase II, and in vitro adhesion and migration assays. The adhesion of swainsonine-treated HCV29 and T24 cells was increased on fibronectin and type IV collagen by 1.5- and 2-fold, respectively, whereas adhesion on laminin was virtually unchanged after swainsonine-treatment in HCV29 cells and was increased in T24 cells. Swainsonine treatment reduced the rate of T24 cell migration by 20%. We concluded that beta1-6 branched tri- and tetraantennary complex-type glycans have an important function in adhesion and migration in the studied cell lines. These data support the view that oligosaccharides are involved in several steps of the metastatic process.
在肿瘤细胞中，细胞糖基化的改变可能在其转移行为中起关键作用。本研究使用了在体内具有非常不同行为的细胞系：HCV29非恶性移行上皮细胞和T24膀胱移行细胞癌。这些行为差异可能部分归因于细胞糖基化模式的差异。使用特异性凝集素对其糖蛋白进行聚糖链分析。通过用高尔基体α-甘露糖苷酶II抑制剂苦马豆素处理这些细胞以及进行体外黏附和迁移试验，研究了碳水化合物的功能作用。苦马豆素处理的HCV29和T24细胞在纤连蛋白和IV型胶原上的黏附分别增加了1.5倍和2倍，而在HCV29细胞中，苦马豆素处理后其在层粘连蛋白上的黏附基本不变，在T24细胞中则增加。苦马豆素处理使T24细胞的迁移率降低了20%。我们得出结论，β1-6分支的三天线和四天线复合型聚糖在所研究的细胞系的黏附和迁移中具有重要功能。这些数据支持了寡糖参与转移过程多个步骤的观点。
