Muller E W, Kim Y, Michael A F, Vernier R L, van der Hem G K, van der Woude F J
Department of Internal Medicine, University Hospital, Groningen, The Netherlands.
Int J Exp Pathol. 1992 Feb;73(1):9-20.
A simple method is presented for selective cell culture of human mesangial cells using explantation of mesangial cell hillocks. Glomeruli which had been incubated with collagenase were explanted on plastic tissue culture flasks. Three to 6 weeks after explantation, a rapidly growing multilayer of elongated mesangial cells was observed to grow over the previously established monolayer of glomerular epithelial cells, ultimately forming multiple nodular foci of mesangial cells or 'mesangial cell hillocks'. By explanting mesangial cell hillocks selectively, pure mesangial cell cultures were easily obtained. When compared with mesangial cells grown in mixed cultures from glomerular explants, the hillock-derived cells were identical in morphology, growth characteristics, cell markers and synthesis of extracellular matrix. This system provides a simple method for the isolation of human mesangial cells in culture.
本文介绍了一种利用系膜细胞小丘外植体进行人系膜细胞选择性细胞培养的简单方法。将用胶原酶孵育过的肾小球外植于塑料组织培养瓶上。外植后3至6周，观察到一层快速生长的多层伸长系膜细胞在先前建立的肾小球上皮细胞单层上生长，最终形成系膜细胞的多个结节状病灶或“系膜细胞小丘”。通过选择性地外植系膜细胞小丘，很容易获得纯系膜细胞培养物。与从肾小球外植体的混合培养物中生长的系膜细胞相比，小丘来源的细胞在形态、生长特性、细胞标志物和细胞外基质合成方面是相同的。该系统为培养中分离人系膜细胞提供了一种简单方法。
