Ziegler Denize R, Gamaro Giovana D, Araújo Emeli, Bassani Marcio G, Perry Marcos Luiz Santos, Dalmaz Carla, Gonçalves Carlos-Alberto
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Ramiro Barcelos, 2600-anexo, 90035-003, Porto Alegre, RS, Brazil.
Physiol Behav. 2005 Mar 16;84(3):421-7. doi: 10.1016/j.physbeh.2005.01.003.
Ketogenic diets have been used to treat epilepsy in children for almost 80 years. However, there are only few studies concerning behavioral effects of these diets, besides their efficacy in treating seizure disorders induced by kainic acid or pentylenetetrazol in rats. Here, rats were fed with a ketogenic diet and locomotion, anxiety and nociception were investigated after 10 weeks. Male Wistar rats were weight matched and divided into two groups: control rats, that received regular laboratory ration, and KD rats, that received ketogenic diet (70% fat, 24% protein and no carbohydrate). Behavioral tests were applied after 10-12 weeks of treatment, and included tests to evaluate exploration (habituation to the open field), anxiety (plus-maze), and nociception (tail-flick measurement). Performance of the animals in the open field revealed a significant difference in the number of crossings, suggesting a higher locomotor activity in animals fed with a ketogenic diet. No differences in anxiety were observed, as evaluated by the plus-maze test. Nociception was measured by the latency in the tail-flick test, and ketogenic rats presented a hypernociceptive response. Yet, these animals responded to a stressor with the classic analgesia, similarly to the controls. The response of ketogenic diet fed rats to the stressor, however, was more prolonged. Exposure to a ketogenic diet may induce higher locomotor activity, together with a hypernociceptive state in the animals, possibly as a result of some alteration in the neural systems involved in the modulation of these behaviors.
生酮饮食用于治疗儿童癫痫已有近80年的历史。然而，除了其对大鼠由海藻酸或戊四氮诱发的癫痫发作障碍的治疗效果外，关于这些饮食对行为影响的研究很少。在此，给大鼠喂食生酮饮食，并在10周后研究其运动、焦虑和痛觉感受。将体重匹配的雄性Wistar大鼠分为两组：对照组大鼠接受常规实验室日粮，生酮饮食组大鼠接受生酮饮食（70%脂肪、24%蛋白质且无碳水化合物）。在治疗10 - 12周后进行行为测试，包括评估探索行为（旷场适应）、焦虑（十字迷宫）和痛觉感受（甩尾测量）的测试。动物在旷场中的表现显示穿越次数存在显著差异，表明喂食生酮饮食的动物具有更高的运动活性。通过十字迷宫测试评估，未观察到焦虑方面的差异。通过甩尾测试中的潜伏期测量痛觉感受，生酮饮食组大鼠呈现出痛觉过敏反应。然而，与对照组类似，这些动物对压力源的反应是经典的镇痛反应。不过，喂食生酮饮食的大鼠对压力源的反应持续时间更长。暴露于生酮饮食可能会使动物产生更高的运动活性以及痛觉过敏状态，这可能是由于参与调节这些行为的神经系统发生了某些改变所致。
