Back Evelyn I, Frindt Claudia, Oćenásková Erika, Nohr Donatus, Stern Martin, Biesalski Hans K
University of Hohenheim, Institute of Biological Chemistry and Nutrition, Garbenstr. 30, 70593 Stuttgart, Germany.
Eur J Nutr. 2006 Feb;45(1):1-6. doi: 10.1007/s00394-005-0556-9. Epub 2005 Mar 22.
Bioavailability of fat-soluble vitamins from conventional oral supplements is insufficient in some conditions in which fat digestion and absorption are chronically impaired (e. g. cystic fibrosis).
We used a water-soluble form of fatsoluble vitamin E (AQUANOVA solubilisate) to create a nutritional supplement (NS) in the form of vitaminized gummi bears (with micellised water-soluble alpha-tocopheryl acetate (100 IU) and 400 mg crystalline vitamin C). We assessed the bioavailability of the NS in comparison to conventional preparations.
The trial consisted of three study days (d0: NS sucked; d10: NS swallowed; d20: reference products swallowed). A total of 14 subjects (6 male/8 female), aged 25.3 (22.7-35.3) years, BMI 24.3 (19.0-31.7) kg/m(2) participated in the study. They had blood samples drawn after fasting for >or=12 hours and then 1, 5, 15, 30, 60, 120, 180, 240, 300 and 320 minutes after ingesting the vitamins. HPLC and a colorimetric method were used to determine vitamin E and vitamin C, respectively. Areas under the curve (AUC(0-320min)) and maximum increases in plasma concentrations (Delta concentration) were calculated to assess bioavailability.
The AUCs(0-320min) of alpha-tocopherol from d0 were significantly larger (p = 0.016) when compared to d20. Moreover, the maximum increase in alpha-tocopherol plasma concentrations was significantly higher for d0 (p = 0.023) and d10 (p = 0.002) when compared to d20.
Short-term bioavailability of AQUANOVA micellised fat-soluble vitamin E from our NS was significantly higher than from regular supplements. The NS will now be tested for its clinical efficacy in a randomized double-blind controlled intervention trial with CF patients.
在脂肪消化和吸收长期受损的某些情况下（如囊性纤维化），传统口服补充剂中脂溶性维生素的生物利用度不足。
我们使用了脂溶性维生素E的水溶性形式（AQUANOVA可溶性制剂）来制作一种营养补充剂（NS），其形式为维生素化小熊软糖（含有微乳化的水溶性醋酸生育酚（100国际单位）和400毫克结晶维生素C）。我们评估了该NS与传统制剂相比的生物利用度。
试验包括三个研究日（第0天：吮吸NS；第10天：吞咽NS；第20天：吞咽参考产品）。共有14名受试者（6名男性/8名女性）参与研究，年龄为25.3（22.7 - 35.3）岁，体重指数为24.3（19.0 - 31.7）kg/m²。他们在禁食≥12小时后采血，然后在摄入维生素后的1、5、15、30、60、120、180、240、300和320分钟采血。分别使用高效液相色谱法和比色法测定维生素E和维生素C。计算曲线下面积（AUC(0 - 320min)）和血浆浓度的最大增加值（Δ浓度）以评估生物利用度。
与第20天相比，第0天α - 生育酚的AUCs(0 - 320min)显著更大（p = 0.016）。此外，与第20天相比，第0天（p = 0.023）和第10天（p = 0.002）α - 生育酚血浆浓度的最大增加值显著更高。
我们的NS中AQUANOVA微乳化脂溶性维生素E的短期生物利用度显著高于常规补充剂。现在将在一项针对囊性纤维化患者的随机双盲对照干预试验中测试该NS的临床疗效。
