采用间接紫外检测的毛细管区带电泳法分离磷脂
Separation of phospholipids by capillary zone electrophoresis with indirect ultraviolet detection.
作者信息
Gao Fei, Dong Juan, Li Wei, Wang Tao, Liao Jie, Liao Yiping, Liu Huwei
机构信息
Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemistry, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
出版信息
J Chromatogr A. 2006 Oct 20;1130(2):259-64. doi: 10.1016/j.chroma.2006.03.070. Epub 2006 Apr 18.
A simple method for separation of different anionic and zwitterionic phospholipid classes by capillary zone electrophoresis (CZE), using indirect UV detection with adenosine monophosphate (AMP) as background electrolyte and the UV-absorbing additive, was successfully developed in this study. The separation conditions including apparent pH (pH*) of running buffer, concentration of AMP, organic solvent, applied voltage and capillary temperature were systematically optimized. The application of this method to human blood sample was also briefly examined.
本研究成功开发了一种简单的方法，通过毛细管区带电泳（CZE）分离不同的阴离子和两性离子磷脂类，使用磷酸腺苷（AMP）作为背景电解质和紫外线吸收添加剂进行间接紫外检测。系统优化了分离条件，包括运行缓冲液的表观pH（pH*）、AMP浓度、有机溶剂、施加电压和毛细管温度。还简要考察了该方法在人体血液样本中的应用。
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