Ribeiro Fabíola Karla Corrêa, Dettoni Valdério do Valle, Peres Renata Lyrio, Vinhas Solange Alves, Có Tatiana Resende, Dietze Reynaldo, Palaci Moisés
Núcleo de Doenças Infecciosas do Centro Biomédico da Universidade Federal do Espirito Santo, Vitória, ES, Brazil.
Rev Soc Bras Med Trop. 2004 Nov-Dec;37(6):431-5. doi: 10.1590/s0037-86822004000600001.
A ligase chain reaction DNA amplification method for direct detection of Mycobacterium tuberculosis (Abbott LCx MTB) in respiratory specimens was evaluated. Results from LCx MTB Assay were compared with those from acid fast bacilli smear, culture, and final clinical diagnosis for each patient. A total of 297 respiratory specimens (sputum and bronchial lavage) from 193 patients were tested. The sensitivity, specificity, positive predictive value and negative predictive value of LC vs culture were 92.7%, 93%, 67.8% and 98.7%, respectively. When compared to the clinical final diagnosis, the sensitivity, specificity, PPV and NPV for LCx were 88.9%, 96.8%, 86.5% and 97.4%, respectively. The sensitivity of LCx MTB assay was 75% for smear-negative, culture positive samples. The results indicate that LCx MTB assay is a rapid, simple and valuable technique as a complementary tool for the diagnosis of tuberculosis.
评估了一种用于直接检测呼吸道标本中结核分枝杆菌的连接酶链反应DNA扩增方法（雅培LCx MTB）。将LCx MTB检测结果与每位患者的抗酸杆菌涂片、培养及最终临床诊断结果进行比较。共检测了193例患者的297份呼吸道标本（痰液和支气管灌洗液）。LC与培养相比的敏感性、特异性、阳性预测值和阴性预测值分别为92.7%、93%、67.8%和98.7%。与临床最终诊断相比，LCx的敏感性、特异性、阳性预测值和阴性预测值分别为88.9%、96.8%、86.5%和97.4%。对于涂片阴性、培养阳性的样本，LCx MTB检测的敏感性为75%。结果表明，LCx MTB检测作为结核病诊断的一种补充工具，是一种快速、简单且有价值的技术。
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