用于呼吸道标本中结核分枝杆菌直接检测的连接酶链反应评估
Evaluation of ligase chain reaction for direct detection of Mycobacterium tuberculosis in respiratory specimens.
作者信息
Denis O, Devaster J M, Vandenberg O, Vanachter H, Lafontaine T, Lin C, Butzler J P
机构信息
Department of Microbiology, Saint-Pierre University Hospital, Brussels, Belgium.
出版信息
Zentralbl Bakteriol. 1998 Jul;288(1):59-65. doi: 10.1016/s0934-8840(98)80099-6.
A new automated amplification method, Ligase Chain Reaction (LCx MTB), was evaluated for direct detection of Mycobacterium tuberculosis in respiratory specimens from 208 patients and its performance was compared with culture and direct smears. Out of 226 specimens, 28 LCx MTB and 15 cultures were found positive for M. tuberculosis. After resolution of clinical history, the sensitivity of LCx MTB and culture was respectively 89.3% and 53.6% with a specificity of 98.5% and 100%. However, samples coming from untreated patients presented similar results between culture and LCx MTB (sensitivity 75% and 83.3% for culture and LCx MTB).
一种新的自动化扩增方法——连接酶链反应（LCx MTB），用于直接检测208例患者呼吸道标本中的结核分枝杆菌，并将其性能与培养法和直接涂片法进行比较。在226份标本中，发现28份LCx MTB检测结果和15份培养结果为结核分枝杆菌阳性。结合临床病史分析后，LCx MTB和培养法的敏感性分别为89.3%和53.6%，特异性分别为98.5%和100%。然而，来自未经治疗患者的样本在培养法和LCx MTB之间呈现出相似的结果（培养法和LCx MTB的敏感性分别为75%和83.3%）。
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