Bardonnet Pierre-Louis, Faivre Vincent, Pirot Fabrice, Boullanger Paul, Falson Françoise
Laboratoire de Pharmacie Galénique Industrielle, EA 3741, ISPB, Université Claude Bernard, Lyon I, 8 av. Rockefeller, 69373 Lyon, France.
Biochem Biophys Res Commun. 2005 Apr 22;329(4):1186-92. doi: 10.1016/j.bbrc.2005.02.092.
Glycosides of cholesteryl oligoethyleneglycols have been synthesized and embedded in liposome bilayers. Several methods as steady-state fluorescence polarization, differential scanning calorimetry, zeta potential, and agglutination have been used to describe the physicochemical outcome of the incorporation of these synthetic glycolipids within phospholipid layers. From calorimetry and fluorescence experiments, it is apparent that the glycolipids decrease the transition temperature of the bilayers in a more important extent than cholesterol. Furthermore, the thickness of the aqueous layer fixed around the liposome is independent of the presence of glycolipids, suggesting that their hydrophilic parts (tetraethyleneglycol spacer and sugar moiety) are not completely extended towards the aqueous phase. Nevertheless, an important and specific interaction has been observed between such glycosylated liposomes and vegetal lectins.
胆固醇基低聚乙二醇糖苷已被合成并嵌入脂质体双层中。几种方法，如稳态荧光偏振、差示扫描量热法、ζ电位和凝集法，已被用于描述这些合成糖脂掺入磷脂层后的物理化学结果。从量热法和荧光实验中可以明显看出，糖脂比胆固醇更能显著降低双层膜的转变温度。此外，脂质体周围固定水层的厚度与糖脂的存在无关，这表明它们的亲水部分（四乙二醇间隔基和糖部分）没有完全伸向水相。然而，已观察到这种糖基化脂质体与植物凝集素之间存在重要的特异性相互作用。
