Babinski M A, deBrito-Gitirana L, Chagas M A, Abidú-Figueiredo M, Costa W S, Sampaio F J B
Urogenital Research Unit, Department of Anatomy, State University of Rio de Janeiro, UERJ, RJ, Brazil.
Anim Reprod Sci. 2005 Apr;86(3-4):317-28. doi: 10.1016/j.anireprosci.2004.08.002.
The purpose of the present study was to verify the smooth muscle cell distribution and elastic system fibers volumetric density (Vv) in the corpus spongiosum and corpus cavernosum of the wild boar penis. Adult wild boars (n=13) were used. The penile mid shaft fragments were fixed with 4% phosphate buffered formalin solution and/or Bouin's liquid during 24-48 h, and processed using standard histological techniques. The sections were stained with Weigert's Resorcin-Fucsin with previous oxidation. The elastic system fibers Vv was determined in 25 random fields of each fragment using M42 test system. For immunohistochemical analysis, monoclonal anti-alpha actin smooth muscle was used. The histochemical methods detected elastic system fibers in both corpus spongiosum and corpus cavernosum of all animals. The elastic fibers Vv average was 36.6%+/-0.9 for corpus spongiosum and 11.7%+/-0.5 for corpus cavernosum. Through immunocitochemistry, a small quantity of smooth muscle cells was visualized in intimate relationship with blood vessels wall. The great amount of elastic fibers and the smooth muscle cell distribution beneath the endothelium suggest that these fibers may have an important role in penile erection process in the penis of wild boars.
本研究的目的是验证野猪阴茎海绵体和海绵体白膜中平滑肌细胞的分布以及弹性系统纤维的体积密度（Vv）。使用了成年野猪（n = 13）。阴茎中段片段在24 - 48小时内用4%磷酸盐缓冲福尔马林溶液和/或Bouin氏液固定，并采用标准组织学技术进行处理。切片先用氧化处理，再用魏格特氏间苯二酚 - 品红染色。使用M42测试系统在每个片段的25个随机视野中测定弹性系统纤维的Vv。免疫组织化学分析采用单克隆抗α肌动蛋白平滑肌抗体。组织化学方法在所有动物的海绵体和海绵体白膜中均检测到弹性系统纤维。海绵体弹性纤维Vv平均值为36.6%±0.9，海绵体白膜为11.7%±0.5。通过免疫细胞化学，可见少量平滑肌细胞与血管壁密切相关。大量的弹性纤维以及内皮下方的平滑肌细胞分布表明，这些纤维可能在野猪阴茎勃起过程中发挥重要作用。
