Fellow Master degree, Postgraduate Program in Surgical Clinic, Universidade Federal do Paraná, Curitiba-PR, Brazil.
Msc, Department of Clinical Surgery, Health Sciences Sector, Universidade Federal do Paraná, Curitiba-PR, Brazil.
Acta Cir Bras. 2021 Jan 20;35(12):e351203. doi: 10.1590/ACB351203. eCollection 2021.
To quantify the age-dependent changes in the relative volume of elastic fibers, collagen fibers and the proportion of collagen types I/III in the corpus spongiosum of rats.
Forty-eight rats, raised under similar conditions, were divided into four groups (G1 to G4) and underwent penectomy at the ages of 6, 9, 12 and 24 months, respectively. Histological sections from the middle segment of the penis were stained with Weigert's resorcin-fuchsin, Masson's trichrome and Picrosirius red, the volumetric density of elastic fibers, collagen fibers and the proportion of collagen types I and III in the corpus spongiosum were determined by stereological analysis.
A reduction in the proportion of collagen I/III between the groups G3 and G4 (p < 0.048) was observed. In the volumetric analysis of elastic fiber, we observed a significant rise between the groups G2 and G3 (p < 0.03) and a reduction of the volume between the groups G3 and G4 (p < 0.01). However, there was no difference in the quantity of total collagen between the groups (p > 0.54).
Aging in rats did not change the quantity of total collagen but reduced the proportion of collagen types I/III and the volume of elastic fibers.
定量研究大鼠海绵体中弹性纤维、胶原纤维的相对体积以及 I/III 型胶原比例随年龄的变化。
将 48 只在相似条件下饲养的大鼠分为 4 组（G1 至 G4），分别在 6、9、12 和 24 月龄时行去势术。取阴茎中段组织行病理切片，分别行 Weigert 氏弹力纤维染色、Masson 三色染色和苦味酸天狼猩红染色，通过体视学分析测量海绵体中弹性纤维、胶原纤维的体积密度以及 I/III 型胶原的比例。
与 G3 组和 G4 组相比，G3 组和 G4 组的 I/III 型胶原比例降低（p<0.048）。弹性纤维的体积分析显示，G2 组和 G3 组之间有显著升高（p<0.03），而 G3 组和 G4 组之间则有显著降低（p<0.01）。然而，各组之间的总胶原含量无差异（p>0.54）。
在大鼠中，衰老不会改变总胶原的含量，但会降低 I/III 型胶原的比例和弹性纤维的体积。
