通过结构域II假定环区的突变增强Cry19Aa对埃及伊蚊的杀蚊活性。
Enhancement of Cry19Aa mosquitocidal activity against Aedes aegypti by mutations in the putative loop regions of domain II.
作者信息
Abdullah Mohd Amir F, Dean Donald H
机构信息
Department of Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
出版信息
Appl Environ Microbiol. 2004 Jun;70(6):3769-71. doi: 10.1128/AEM.70.6.3769-3771.2004.
Abstract
Improvements in the mosquitocidal activity of Bacillus thuringiensis Cry19Aa were achieved by protein engineering of putative surface loop residues in domain II through rational design. The improvement of Aedes toxicity in Cry19Aa was 42,000-fold and did not affect its toxicity against Anopheles or Culex.
摘要
通过合理设计对苏云金芽孢杆菌Cry19Aa结构域II中假定的表面环残基进行蛋白质工程改造，实现了其杀蚊活性的提高。Cry19Aa对伊蚊的毒性提高了42000倍，且不影响其对按蚊或库蚊的毒性。
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本文引用的文献
1
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Appl Environ Microbiol. 2003 Sep;69(9):5343-53. doi: 10.1128/AEM.69.9.5343-5353.2003.
2
Lack of cross-resistance to Cry19A from Bacillus thuringiensis subsp. jegathesan in Culex quinquefasciatus (Diptera: Culicidae) resistant to cry toxins from Bacillus thuringiensis subsp. israelensis.
Appl Environ Microbiol. 2001 Apr;67(4):1956-8. doi: 10.1128/AEM.67.4.1956-1958.2001.
3
Enhanced toxicity of Bacillus thuringiensis Cry3A delta-endotoxin in coleopterans by mutagenesis in a receptor binding loop.
FEBS Lett. 2000 May 12;473(2):227-32. doi: 10.1016/s0014-5793(00)01505-2.
4
Bacillus thuringiensis and its pesticidal crystal proteins.
Microbiol Mol Biol Rev. 1998 Sep;62(3):775-806. doi: 10.1128/MMBR.62.3.775-806.1998.
5
SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling.
Electrophoresis. 1997 Dec;18(15):2714-23. doi: 10.1002/elps.1150181505.
6
Contribution of the 65-kilodalton protein encoded by the cloned gene cry19A to the mosquitocidal activity of Bacillus thuringiensis subsp. jegathesan.
Appl Environ Microbiol. 1997 Nov;63(11):4449-55. doi: 10.1128/aem.63.11.4449-4455.1997.
7
Probing the mechanism of action of Bacillus thuringiensis insecticidal proteins by site-directed mutagenesis--a minireview.
Gene. 1996 Nov 7;179(1):111-7. doi: 10.1016/s0378-1119(96)00442-8.
8
ProMod and Swiss-Model: Internet-based tools for automated comparative protein modelling.
Biochem Soc Trans. 1996 Feb;24(1):274-9. doi: 10.1042/bst0240274.
9
Characterization of six highly mosquitocidal Bacillus thuringiensis strains that do not belong to H-14 serotype.
Curr Microbiol. 1996 Jan;32(1):48-54. doi: 10.1007/s002849900009.
10
Identification of amino acid residues of Bacillus thuringiensis delta-endotoxin CryIAa associated with membrane binding and toxicity to Bombyx mori.
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