Sanicola Shawn M, Albert Stephen F
Denver Veterans Administration Medical Center, Lakewood, CO, USA.
J Foot Ankle Surg. 2005 Mar-Apr;44(2):121-4. doi: 10.1053/j.jfas.2005.01.006.
The purpose of this study was to determine the elution characteristics of vancomycin and tobramycin when mixed with calcium sulfate to form antibiotic beads. Calcium sulfate was combined with vancomycin and tobramycin separately to form 2 types of antibiotic beads, which were packaged and labeled separately. The packaged calcium sulfate beads with vancomycin and tobramycin were then gas sterilized. The beads were placed in phosphate-buffered saline and kept at 36 degrees C for 6 weeks. Two separate series of assays were run simultaneously for both types of beads. In one assay, a bead containing vancomycin was placed in a fresh vial of phosphate buffered saline after each assay. The same was done with beads containing tobramycin. In the second series of assays, 9 vials of phosphate buffered saline each containing 1 vancomycin bead and 9 vials of phosphate buffered saline each containing 1 tobramycin bead was arranged. The phosphate-buffered saline was then assayed at predetermined times for both the vancomycin bead series and the tobramycin bead series. The amount of vancomycin and tobramycin assayed nearly equaled the calculated amount of antibiotic per bead measured before bead construction. Also, the elution of antibiotic from the calcium sulfate was complete within 72 hours. In conclusion, the construction and gas sterilization of calcium sulfate beads containing vancomycin and tobramycin does not destroy vancomycin and tobramycin. Also, the complete elution of available vancomycin and tobramycin in calcium sulfate beads occurs within 72 hours.
本研究的目的是确定万古霉素和妥布霉素与硫酸钙混合形成抗生素珠时的洗脱特性。硫酸钙分别与万古霉素和妥布霉素结合，形成2种类型的抗生素珠，并分别进行包装和标记。然后对包装好的含万古霉素和妥布霉素的硫酸钙珠进行气体灭菌。将珠子置于磷酸盐缓冲盐水中，在36℃下保存6周。对两种类型的珠子同时进行两个独立系列的测定。在一项测定中，每次测定后将含万古霉素的珠子放入一新瓶磷酸盐缓冲盐水中。含妥布霉素的珠子也进行同样操作。在第二系列测定中，安排9瓶磷酸盐缓冲盐水，每瓶含1颗万古霉素珠，以及9瓶磷酸盐缓冲盐水，每瓶含1颗妥布霉素珠。然后在预定时间对万古霉素珠系列和妥布霉素珠系列的磷酸盐缓冲盐水进行测定。所测定的万古霉素和妥布霉素的量几乎等于珠子制作前每颗珠子计算的抗生素量。此外，硫酸钙中抗生素的洗脱在72小时内完成。总之，含万古霉素和妥布霉素的硫酸钙珠的制作和气体灭菌不会破坏万古霉素和妥布霉素。而且，硫酸钙珠中可用的万古霉素和妥布霉素在72小时内完全洗脱。
