McKeown Sonja J, Lee Vivian M, Bronner-Fraser Marianne, Newgreen Donald F, Farlie Peter G
Embryology Laboratory, Murdoch Children's Research Institute, Royal Children's Hospital, and Department of Paediatrics, University of Melbourne, Parkville, Victoria, Australia.
Dev Dyn. 2005 Jun;233(2):430-44. doi: 10.1002/dvdy.20341.
SoxE genes (Sox8, Sox9, and Sox10) are early response genes to neural crest induction. Although the early role of Sox9 has been examined in chick and frog, later roles in neural crest migration and differentiation remain largely unexplored. We first examined which SoxE genes were expressed in trunk neural crest cells and then investigated their function using in ovo electroporation. The results of this analysis reveal that Sox10 is present in migrating neural crest cells, whereas other SoxE genes are only expressed transiently after induction. Ectopic expression of Sox10 in the neural tube at trunk level induced expression of HNK-1 in neuroepithelial cells followed by extensive emigration from all levels of the dorsoventral neuraxis, including the floor plate. Sox10-expressing cells failed to express neuronal, Schwann, or melanocyte markers up to 6 days posttransfection (E8), suggesting these cells were maintained in an undifferentiated state. Overexpression of Sox8 or Sox9 had similar but not identical effects on neuroepithelial cells. These results show that high levels of Sox10, Sox9, or Sox8 expression in the neural tube are capable of inducing a migratory neural crest-like phenotype even in the absence of dorsal signals and can maintain these cells in an undifferentiated state.
SoxE基因（Sox8、Sox9和Sox10）是神经嵴诱导的早期反应基因。尽管已在鸡和蛙中研究了Sox9的早期作用，但在神经嵴迁移和分化中的后期作用仍 largely unexplored。我们首先检测了哪些SoxE基因在躯干神经嵴细胞中表达，然后使用卵内电穿孔法研究它们的功能。该分析结果表明，Sox10存在于迁移的神经嵴细胞中，而其他SoxE基因仅在诱导后短暂表达。在躯干水平的神经管中异位表达Sox10会诱导神经上皮细胞中HNK-1的表达，随后包括底板在内的背腹神经轴各水平均出现广泛的细胞迁出。直至转染后6天（E8），表达Sox10的细胞仍未表达神经元、雪旺氏细胞或黑素细胞标志物，这表明这些细胞维持在未分化状态。Sox8或Sox9的过表达对神经上皮细胞有相似但不完全相同的影响。这些结果表明，即使在没有背侧信号的情况下，神经管中高水平的Sox10、Sox9或Sox8表达也能够诱导出类似迁移神经嵴的表型，并能使这些细胞维持在未分化状态。
