Wang Wu-Qing, Wang Zheng-Min, Chi Fang-Lu
Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai, People's Republic of China.
Acta Otolaryngol. 2004 Dec;124(10):1141-4. doi: 10.1080/00016480410022921.
To investigate the spontaneous healing process of various types of perforation and the location of the epithelial generation center in the tympanic membrane of rats.
Various types of perforation were made in the ears of 50 rats and the healing process was observed using light and electron microscopy.
Epithelia hyperplasia could not be seen at the edge of the perforations, but occurred in the annulus and handle of malleus regions during the early stage of healing. There was no correlation between the healing time and the size of the perforations. When one ear was perforated, no changes were observed in the intact ear on the other side. The epithelium did not migrate into the tympanic cavity in the posterior marginal perforations. All the perforations healed, although the manubria of the malleus were damaged.
The perforations healed by means of epithelial migration. The epithelial generation center was located near the annulus and the handle of the malleus, and therefore protection of these two regions is very important in middle ear surgery.
研究大鼠鼓膜不同类型穿孔的自然愈合过程以及上皮生成中心的位置。
在50只大鼠的耳部制造不同类型的穿孔，并用光学显微镜和电子显微镜观察愈合过程。
在穿孔边缘未见上皮增生，但在愈合早期，上皮增生出现在鼓环和锤骨柄区域。愈合时间与穿孔大小之间无相关性。当一侧耳穿孔时，另一侧完整耳未观察到变化。后缘穿孔时上皮未迁移至鼓室。尽管锤骨柄受损，但所有穿孔均愈合。
穿孔通过上皮迁移愈合。上皮生成中心位于鼓环和锤骨柄附近，因此在中耳手术中保护这两个区域非常重要。
