[抑制化学发光检测流动注射分析法测定痕量强力霉素]

[Flow injection analysis of trace amounts of doxycycline with inhibited chemiluminescence detection].

作者信息

Li Nain-bing, Duan Jian-ping, Chen Hong-qing, Chen Guo-nan

机构信息

College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2004 Jan;24(1):15-7.

PMID:15768965

Abstract

A novel flow injection chemiluminescence method has been developed for the determination of doxycycline (DC) based on the inhibition of chemiluminescence reaction of Luminol-KMnO4 by using DC in sodium hydroxide medium. Using different concentrations of KMnO4, a series of good linear regression equations of decreased chemiluminescence intensity and the concentration of DC were obtained with different subsections in the concentration range of 0.005-5.0 microg x mL(-1). The detection limit (3sigma) is 2.0 x 10(-3) microg x mL(-1). The method has been successfully applied to the determination of DC in a pharmaceutical preparation.

摘要

基于在氢氧化钠介质中利用强力霉素（DC）对鲁米诺-高锰酸钾化学发光反应的抑制作用，开发了一种新型流动注射化学发光法用于测定强力霉素（DC）。使用不同浓度的高锰酸钾，在0.005 - 5.0微克×毫升⁻¹的浓度范围内，不同子区间获得了一系列化学发光强度降低值与DC浓度的良好线性回归方程。检测限（3σ）为2.0×10⁻³微克×毫升⁻¹。该方法已成功应用于药物制剂中DC的测定。