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利用 DNA、蛋白质和形态学标记对荞麦进行遗传作图。

Genetic mapping of common buckwheat using DNA, protein and morphological markers.

机构信息

Institute of Plant Genetics and Breeding, School of Life Science, Guizhou Normal University, Guiyang, PR China.

出版信息

Hereditas. 2010 Feb;147(1):27-33. doi: 10.1111/j.1601-5223.2009.02116.x.

DOI:10.1111/j.1601-5223.2009.02116.x
PMID:20416014
Abstract

Genetic mapping of F(2) progeny (n = 225) of hybrids between the Sobano variety of common buckwheat (Fagopyrum esculentum Moench) and the Homo wild accession (F. esculentum var. homotropicum) was carried out using randomly amplified polymorphic DNA (RAPD), sequence-tagged site (STS) and seed protein subunit markers, and three morphological traits. Ten linkage groups were identified, involving 87 RAPD markers, 12 STS markers, four seed protein subunit (PS(62)/PS(59), PS(49.8)/PS(51.4), PS(44)/PS(42.9), and PS(39.9)/PS(37.8)) markers, and three morphological alleles controlling homo/long style (H/s), shattering habit (Sht/sht), and acute/obtuse achene ridge (Ac/ac), covering a total of 655.2 cM.

摘要

利用随机扩增多态性 DNA(RAPD)、序列标记位点(STS)和种子蛋白亚基标记以及 3 个形态性状,对甜荞栽培品种(Fagopyrum esculentum Moench)苏巴诺品系与野生种(F. esculentum var. homotropicum)杂交 F2 后代(n=225)进行了遗传作图。鉴定出 10 个连锁群,涉及 87 个 RAPD 标记、12 个 STS 标记、4 个种子蛋白亚基(PS(62)/PS(59)、PS(49.8)/PS(51.4)、PS(44)/PS(42.9)和 PS(39.9)/PS(37.8))标记和 3 个控制同型/长花柱(H/s)、易落粒(Sht/sht)和锐/钝颖果脊(Ac/ac)的形态等位基因,共覆盖 655.2cM。

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