Li Dong-mei, Yu Dong-zheng, Liu Qi-yong, Gong Zheng-da
Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2004 Nov;25(11):934-7.
To investigate Bartonella infections in small mammalian reservoir hosts from different environments and types of climate in Yunnan.
Femoral blood samples were collected from the anesthetic captured animals from five counties including three types of climate. All isolates were grown on brain and heart infusion agar plates containing 5% defibrinated rabbit blood. The agar plates were incubated at 35 degrees C in a humidified with 5% CO2 environment for at least 4 weeks. Bartonella-like isolates were confirmed by the polymerase chain reaction and visualizing the target gene fragment by gel electrophoresis.
Bartonella species were isolated from 69 of 176 small animals including 4 species of 3 genera from 4 counties and the total prevalence in rodents was 39.2%. The maximal prevalence was 42.0% of Rattus tanezumi flavipectus usually inhabiting indoors and courtyard and contacting closely to human. Moreover, Bartonella isolates were obtained from Rattus noruegicus, Eothenomys miletus and Mus pahari. Life environments of captured animals involved indoors, courtyard, brush and forest in mountain.
The finding in this study suggested the characteristic of diversity of Bartonella infections in rodent hosts in southern China included Bartonella species parasiting in a wide range of animal hosts in different environments as well as climate types. Further investigations were needed in different areas in China to confirm more mammalian reservoir hosts with Bartonella infections.
调查云南不同环境和气候类型的小型哺乳动物储存宿主中的巴尔通体感染情况。
从包括三种气候类型的五个县的麻醉捕获动物中采集股血样本。所有分离株均在含有5%去纤维兔血的脑心浸液琼脂平板上培养。琼脂平板在35℃、5%二氧化碳加湿的环境中孵育至少4周。通过聚合酶链反应和凝胶电泳可视化目标基因片段来确认巴尔通体样分离株。
从176只小动物中的69只分离出巴尔通体菌种，包括来自4个县的3属4种，啮齿动物中的总患病率为39.2%。患病率最高的是通常栖息在室内和庭院且与人类密切接触的黄胸鼠，为42.0%。此外，还从褐家鼠、大绒鼠和锡金小家鼠中获得了巴尔通体分离株。捕获动物的生活环境包括室内、庭院、灌木丛和山区森林。
本研究结果表明，中国南方啮齿动物宿主中巴尔通体感染具有多样性特征，包括巴尔通体菌种寄生于不同环境和气候类型的多种动物宿主。中国不同地区需要进一步调查，以确认更多感染巴尔通体的哺乳动物储存宿主。
