[福建沿海地区鼠类宿主中巴尔通体菌种的流行情况及基因型研究]

[Study on the prevalence and genotypes of Bartonella species in rodent hosts from Fujian coastal regions].

作者信息

Ye Xi, Li Guo-wei, Yao Mei-lin, Luo Wei, Su Li-qiong

机构信息

Xiamen Haicang Center for Disease Control and Prevention, Xiamen 361026, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2009 Oct;30(10):989-92.

PMID:20193373

Abstract

OBJECTIVE

To investigate Bartonella infection in rodent hosts from different environments and types of climate in Fujian coastal regions. Genetypes of the Bartonella strains was also studied to provide scientific basis for prevention and control of the correlated diseases.

METHODS

By random sampling method, we selected six study sites in Fujian southeastern coastal regions. Rodents were captured by cages to Isolate Bartonella strains. Bartonella-like isolates were confirmed by polymerase chain reaction (PCR). The 379 bp fragment of gltA gene was sequenced and the growth and development tree was constructed to determine Bartonella species. Distribution of Bartonella species in the different area and related hosts was also analysed.

RESULTS

Bartonella species were isolated from 188 of 1161 small animals including five rodent species. The infected animals were grouped into 2 genera and 2 orders. They were Suncus murinus, Rattus norvegicus, Rattus flavipectus, Mus musculus and Rattus rattus. The overall prevalence of Bartonella bacteremia was 16.19% in the most prevalent species of rodents in Fujian southeastern coastal regions including 21.43% in Suncus murinus, 13.54% in Rattus norvegicus and 18.27% in Rattus flavipectus. Rodents in every investigated areas were infected by Bartonella species (9.25% in Ningde, 9.52% in Fuzhou, 9.38% in Putian, 28.18% in Quanzhou, 17.42% in Xiamen and 13.33% in Zhangzhou). There were significant differences among infected rates in different annual accumulated temperature districts (chi(2) = 12.93, P < 0.001). Isolates from rodents were clustered in three genotypes (B. elizabethae, B. qeenslandensis and B. tribocorum A, B).

CONCLUSION

The local rodents in Fujian southeastern coastal regions were widely infected by Bartonella spp. Differences among the prevalent species of Bartonella in Fujian southeastern coastal region, Yunan and Beijing were noticed. Our findings suggested there was a need to study the prevalence, related vectors and the molecular organism of Bartonella spp.

摘要

目的

调查福建沿海地区不同环境及气候类型啮齿动物宿主中的巴尔通体感染情况，并研究巴尔通体菌株的基因型，为相关疾病的防控提供科学依据。

方法

采用随机抽样法，在福建东南沿海地区选取6个研究地点，用鼠笼捕获啮齿动物以分离巴尔通体菌株，通过聚合酶链反应（PCR）确认疑似巴尔通体分离株。对gltA基因的379bp片段进行测序并构建发育树以确定巴尔通体种类，同时分析不同地区及相关宿主中巴尔通体种类的分布情况。

结果

从1161只小动物中的188只分离到巴尔通体，包括5种啮齿动物。感染动物分属2目2科，分别为臭鼩鼱、褐家鼠、黄胸鼠、小家鼠和黑家鼠。福建东南沿海地区最常见的啮齿动物中巴尔通体菌血症总体患病率为16.19%，其中臭鼩鼱为21.43%，褐家鼠为13.54%，黄胸鼠为18.27%。各调查地区的啮齿动物均有巴尔通体感染（宁德9.25%，福州9.52%，莆田9.38%，泉州28.18%，厦门17.42%，漳州13.33%）。不同年积温区的感染率存在显著差异（χ² = 12.93，P < 0.001）。从啮齿动物分离的菌株聚为3个基因型（伊丽莎白巴尔通体、昆士兰巴尔通体和 tribocorum A、B 型巴尔通体）。