Bianchin Claire, Mauxion Fabienne, Sentis Stéphanie, Séraphin Bertrand, Corbo Laura
Equipe Labellisée La Ligue, Centre de Génétique Moléculaire, CNRS UPR2167 Associée à l'Université Pierre et Marie Curie, 91198 Gif sur Yvette, France.
RNA. 2005 Apr;11(4):487-94. doi: 10.1261/rna.7135305.
The yeast Pop2 protein, belonging to the eukaryotic Caf1 family, is required for mRNA deadenylation in vivo. It also catalyzes poly(A) degradation in vitro, even though this property has been questioned. Caf1 proteins are related to RNase D, a feature supported by the recently published structure of Pop2. Yeast Pop2 contains, however, a divergent active site while its human homologs harbor consensus catalytic residues. Given these differences, we tested whether its deadenylase activity is conserved in the human homologs Caf1 and Pop2. Our data demonstrate that both human factors degrade poly(A) tails indicating their involvement in mRNA metabolism.
酵母Pop2蛋白属于真核生物Caf1家族，是体内mRNA去腺苷酸化所必需的。它在体外也能催化聚腺苷酸（poly(A)）的降解，尽管这一特性存在争议。Caf1蛋白与核糖核酸酶D（RNase D）相关，最近发表的Pop2结构支持了这一特征。然而，酵母Pop2含有一个不同的活性位点，而其人类同源物则具有保守的催化残基。鉴于这些差异，我们测试了其去腺苷酸酶活性在人类同源物Caf1和Pop2中是否保守。我们的数据表明，这两种人类因子都能降解聚腺苷酸尾巴，表明它们参与了mRNA代谢。
