Hansman G S, Natori K, Ushijima H, Katayama K, Takeda N
Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
Arch Virol. 2005 Jul;150(7):1433-7. doi: 10.1007/s00705-005-0506-0. Epub 2005 Mar 17.
Sapovirus (SaV), a member of the genus Sapovirus in the family Caliciviridae, is an agent of human and porcine gastroenteritis. SaV strains were recently divided into five genogroups (GI to GV). We characterized novel polyclonal antibodies raised against SaV GV virus-like particles (VLPs) by Western blot analysis, and both antibody and antigen enzyme-linked immunosorbent assays (ELISAs). Our results have indicated SaV GI and GV VLPs were antigenically distinct by Western blotting and ELISAs. These reagents may be useful for genogroup specific detection of SaV.
札幌病毒（SaV）是杯状病毒科札幌病毒属的成员，是引起人和猪肠胃炎的病原体。最近，SaV毒株被分为五个基因群（GI至GV）。我们通过蛋白质免疫印迹分析以及抗体和抗原酶联免疫吸附测定（ELISA）对针对SaV GV病毒样颗粒（VLP）产生的新型多克隆抗体进行了表征。我们的结果表明，通过蛋白质免疫印迹和ELISA检测，SaV GI和GV VLP在抗原性上存在差异。这些试剂可能有助于对SaV进行基因群特异性检测。
