Hansman G S, Guntapong R, Pongsuwanna Y, Natori K, Katayama K, Takeda N
Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
Arch Virol. 2006 Mar;151(3):551-61. doi: 10.1007/s00705-005-0630-x. Epub 2005 Sep 12.
Human sapovirus (SaV) strains are etiological agents of mild and/or acute gastroenteritis in children and adults. In this study, we describe the development of a novel antigen enzyme-linked immunosorbent assay (ELISA) detection system that was based on hyperimmune rabbit and guinea pig antisera raised against SaV genogroup I (GI) virus-like particles. The ELISA had 100% specificity, and sensitivities of 60% and 25% when compared to single-round PCR and nested PCR, respectively. Our results have shown the ELISA was useful in detecting SaV GI antigens in clinical stool specimens collected two days after the onset of illness.
人札幌病毒（SaV）毒株是儿童和成人轻度和/或急性胃肠炎的病原体。在本研究中，我们描述了一种新型抗原酶联免疫吸附测定（ELISA）检测系统的开发，该系统基于针对SaV基因组I（GI）病毒样颗粒产生的超免疫兔和豚鼠抗血清。该ELISA具有100%的特异性，与单轮PCR和巢式PCR相比，灵敏度分别为60%和25%。我们的结果表明，该ELISA可用于检测发病两天后采集的临床粪便标本中的SaV GI抗原。
