Bahr Janice M, Nakai Masaaki, Rivera Alexander, Walsh Jane, Evans Glenda L, Lotinun Sutada, Turner Russell T, Black Molly, Jeffery Elizabeth H
Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA.
Menopause. 2005 Mar;12(2):165-73. doi: 10.1097/00042192-200512020-00011.
The present study was conducted to determine the effects of dietary soy protein and isoflavones on bone and the reproductive tract in the absence of the ovary.
Three-month-old Sprague-Dawley rats (n = 56) were either sham-operated or ovariectomized and then fed diets containing casein or soy protein +/- isoflavone extract for 12 weeks. The amounts of casein, soy protein, and extract (per kg diet) in each group were as follows: (1) Ovariectomy, 200 g of casein; (2) Ovariectomy+low soy, 100 g of casein + 100 g of soy protein; (3) Ovariectomy+high soy, 200 g of soy protein; (4) Ovariectomy+low extract, 200 g of casein + 17.2 g of extract; (5) Ovariectomy+high extract, 200 g of casein + 34.4 g of extract; (6) Ovary intact, 200 g of casein; (7) Ovariectomy+estradiol-17beta, 200 g of casein. Diet consumption, body weight, uterine weight, urine deoxypyridinoline, and bone mineral density of the femur and lumbar vertebrae were measured. The femur rigidity was evaluated by histomorphometry. The reproductive tract (uterus, vagina, and cervix) was studied histologically.
The Ovariectomy group showed significant increases in body weight, diet consumption, and deoxypyridinoline, decreases in uterine weight and bone mineral density, and negative changes in histomorphometry compared with the Ovary intact group. Neither soy protein nor extract diets abrogated these alterations, except for the Ovariectomy+high extract group that showed statistically significant positive changes in histomorphometric parameters. There were no histological differences in the reproductive tract among Ovariectomy, Ovariectomy+soy, and Ovariectomy+extract groups. The estradiol-17beta replacement abrogated ovariectomy-induced alterations.
Dietary intake of isoflavones by sexually mature ovariectomized rats has a minimal beneficial effect on bone with no effect on the reproductive tract.
本研究旨在确定在卵巢缺失的情况下，膳食大豆蛋白和异黄酮对骨骼及生殖道的影响。
将3月龄的Sprague-Dawley大鼠（n = 56）进行假手术或卵巢切除，然后给予含有酪蛋白或大豆蛋白±异黄酮提取物的饲料，持续12周。每组饲料中酪蛋白、大豆蛋白和提取物的含量（每千克饲料）如下：（1）卵巢切除组，200克酪蛋白；（2）卵巢切除+低大豆组，100克酪蛋白 + 100克大豆蛋白；（3）卵巢切除+高大豆组，200克大豆蛋白；（4）卵巢切除+低提取物组，200克酪蛋白 + 17.2克提取物；（5）卵巢切除+高提取物组，200克酪蛋白 + 34.4克提取物；（6）卵巢完整组，200克酪蛋白；（7）卵巢切除+雌二醇-17β组，200克酪蛋白。测量饲料消耗量、体重、子宫重量、尿脱氧吡啶啉以及股骨和腰椎的骨密度。通过组织形态计量学评估股骨硬度。对生殖道（子宫、阴道和宫颈）进行组织学研究。
与卵巢完整组相比，卵巢切除组的体重、饲料消耗量和脱氧吡啶啉显著增加，子宫重量和骨密度降低，组织形态计量学出现负面变化。除卵巢切除+高提取物组在组织形态计量学参数上显示出统计学上显著的正向变化外，大豆蛋白和提取物饲料均未消除这些改变。卵巢切除组、卵巢切除+大豆组和卵巢切除+提取物组的生殖道在组织学上无差异。雌二醇-17β替代消除了卵巢切除引起的改变。
性成熟的卵巢切除大鼠膳食摄入异黄酮对骨骼有极小的有益作用，对生殖道无影响。
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