Li Mei-Gen, Ding Guo-Lian, Chen Xi-Jing, Lu Xi-Ping, Dong Li-Jun, Dong Min-Yue, Yang Xiao-Fu, Lu Xiu-E, Huang He-Feng
Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, 2 Xue Shi Road, Hangzhou, Zhejiang, 31006, China.
J Clin Endocrinol Metab. 2007 Dec;92(12):4771-6. doi: 10.1210/jc.2007-0978. Epub 2007 Sep 25.
Our objective was to evaluate whether polycystic ovarian syndrome (PCOS)-associated infertility is related to alterations of leptin, leptin receptor (Ob-R), and the phosphorylated signal transducer and activator of transcription 3 (p-STAT3)/suppressor of cytokine signal 3 (SOCS3) system in the ovary.
A case-control study was conducted in a university hospital.
Thirty-one infertile PCOS women with oligoovulation plus polycystic ovarian morphology and 79 infertile women with tubal blockage (control) participated in the study. The subjects were stratified according to in vitro fertilization outcomes: successful and failed subgroups.
Serum and follicular fluid (FF) leptin levels were measured with ELISA. RT-PCR and Western blotting were performed to assess expression of mRNA encoding leptin and Ob-R and proteins of p-STAT3 and SOCS3 in granulosa cells (GCs).
Leptin levels in serum and FF of PCOS women were significantly higher than those of control (P < 0.01). There were no significant differences in expression of leptin mRNA and short and long Ob-Rs between PCOS and control (P > 0.05). The p-STAT3 level was decreased in PCOS compared with control (P < 0.01), whereas SOCS3 remained significantly unchanged (P > 0.05). Further analysis showed that serum and FF leptin levels were significantly higher, whereas p-STAT3 in GCs was lower in the failed subgroup of PCOS than those in the successful subgroup of PCOS (P < 0.05).
Hyperleptinemia and high FF leptin are important pathologies of PCOS with infertility. Lower levels of p-STAT3 in GCs may be related to ovarian leptin resistance and fecundity in PCOS women. Relatively high serum and FF leptin and low p-STAT3 in GCs may account for decreased fertilization, implantation, and pregnancy rates of in vitro fertilization in PCOS women.
我们的目的是评估多囊卵巢综合征（PCOS）相关的不孕症是否与卵巢中瘦素、瘦素受体（Ob-R）以及磷酸化信号转导和转录激活因子3（p-STAT3）/细胞因子信号抑制因子3（SOCS3）系统的改变有关。
在一家大学医院进行了一项病例对照研究。
31例患有排卵少合并多囊卵巢形态的PCOS不孕女性和79例输卵管堵塞的不孕女性（对照组）参与了研究。根据体外受精结果将受试者分为成功和失败亚组。
用酶联免疫吸附测定法（ELISA）测量血清和卵泡液（FF）中的瘦素水平。进行逆转录聚合酶链反应（RT-PCR）和蛋白质印迹法以评估颗粒细胞（GCs）中编码瘦素和Ob-R的mRNA以及p-STAT3和SOCS3蛋白的表达。
PCOS女性血清和FF中的瘦素水平显著高于对照组（P < 0.01）。PCOS组和对照组之间瘦素mRNA以及短、长Ob-Rs的表达无显著差异（P > 0.05）。与对照组相比，PCOS组中p-STAT3水平降低（P < 0.01），而SOCS3仍无显著变化（P > 0.05）。进一步分析表明，PCOS失败亚组的血清和FF瘦素水平显著高于成功亚组，而GCs中的p-STAT3水平低于成功亚组（P < 0.05）。
高瘦素血症和高FF瘦素是PCOS合并不孕症的重要病理特征。GCs中较低水平的p-STAT3可能与PCOS女性的卵巢瘦素抵抗和生育力有关。GCs中相对较高的血清和FF瘦素以及较低的p-STAT3可能是PCOS女性体外受精的受精、着床和妊娠率降低的原因。
