Dayer Dian, Nikbakht Roshan, Kadkhodai Elyaderani Manijeh
Department of Laboratory Sciences, Para Medical Faculty, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
.Department of Obstetrics and Gynecology, Fertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Iran J Reprod Med. 2013 Dec;11(12):1033-4.
Some studies propose that changes in leptin concentrations (above or under the normal range) result in infertility. Therefore, we investigated serum and follicular fluid leptin concentrations in infertile women with polycystic ovary syndrome (PCOS).
To study serum and follicular fluid leptin concentrations in infertile women with PCOS.
We conducted a case-control study. The case group consisted of 30 infertile women with PCOS who were admitted to the Infertility Department of Imam Khomainy Hospital in Ahvaz, Iran. The control group consisted of 30 healthy fertile women adjusted for age and body mass index (BMI) with the case group. On day 14 of the menstrual cycle, 5 ml of blood was obtained from subjects in both groups. Serum and follicular fluid leptin concentrations were determined by the enzyme linked immunosorbent assay (ELISA). A Biovendor kit was used for the measurement of leptin concentrations. All data were analyzed using statistical package for the social sciences (SPSS) software (version 17.0, Nie, Bent & Hull, USA).
There was a significant correlation between BMI and serum leptin concentrations in both the control (p=0.005, r=0.516) and case groups (p=0.006, r=0.547). In the case group, serum leptin concentrations were consistent with follicular fluid leptin concentrations (p<0.001, r=0.839). Comparison of serum leptin concentrations between the case and control groups revealed no significant difference (p=0.56).
Infertility among women with PCOS was not a consequence of changes in leptin concentrations.
一些研究表明，瘦素浓度的变化（高于或低于正常范围）会导致不孕。因此，我们调查了多囊卵巢综合征（PCOS）不孕女性的血清和卵泡液瘦素浓度。
研究PCOS不孕女性的血清和卵泡液瘦素浓度。
我们进行了一项病例对照研究。病例组由30例PCOS不孕女性组成，她们被收治于伊朗阿瓦士伊玛目霍梅尼医院不孕科。对照组由30名健康的有生育能力的女性组成，这些女性在年龄和体重指数（BMI）方面与病例组相匹配。在月经周期的第14天，从两组受试者中采集5毫升血液。采用酶联免疫吸附测定（ELISA）法测定血清和卵泡液瘦素浓度。使用Biovendor试剂盒测量瘦素浓度。所有数据均使用社会科学统计软件包（SPSS）软件（版本17.0，美国Nie、Bent和Hull公司）进行分析。
在对照组（p = 0.005，r = 0.516）和病例组（p = 0.006，r = 0.547）中，BMI与血清瘦素浓度之间均存在显著相关性。在病例组中，血清瘦素浓度与卵泡液瘦素浓度一致（p < 0.001，r = 0.839）。病例组与对照组血清瘦素浓度的比较显示无显著差异（p = 0.56）。
PCOS女性的不孕并非瘦素浓度变化所致。
