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大肠杆菌K-12中脂多糖核心合成相关基因的遗传分析：rfa位点的三个操纵子

Genetic analysis of the genes involved in synthesis of the lipopolysaccharide core in Escherichia coli K-12: three operons in the rfa locus.

作者信息

Roncero C, Casadaban M J

机构信息

Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.

出版信息

J Bacteriol. 1992 May;174(10):3250-60. doi: 10.1128/jb.174.10.3250-3260.1992.

DOI:10.1128/jb.174.10.3250-3260.1992
PMID:1577693
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC205993/
Abstract

The region of the Escherichia coli K-12 chromosome encoding the enzymes responsible for the synthesis of responsible for the synthesis of the lipopolysaccharide (LPS) core has been cloned in vivo by using a mini-Mu vector. This region, formerly known as the rfa locus, comprises 18 kb of DNA between the markers tdh and rpmBG. Results of in vitro mutagenesis of this region with MudII1734 indicate the presence of at least 17 open reading frames or genes, a number considerably higher than expected on the basis of genetic and biochemical studies. Specific insertions in different genes have been recombined into the chromosome, and the mutations have been phenotypically characterized. Complementation analysis indicates that these genes are arranged in three different operons transcribed in opposite directions. A detailed physical map of this region has been constructed on the basis of complementation analysis, fusion protein data, and phenotypic characterizations. Additionally, the role of some genes in the synthesis of LPS has been defined by complementation analysis with known Salmonella typhimurium LPS mutants. The genetic organization of this locus seems to be identical in E. coli K-12 and S. typhimurium.

摘要

利用mini-Mu载体已在体内克隆了大肠杆菌K-12染色体中编码负责脂多糖（LPS）核心合成的酶的区域。该区域以前称为rfa位点，位于标记tdh和rpmBG之间，包含18 kb的DNA。用MudII1734对该区域进行体外诱变的结果表明，至少存在17个开放阅读框或基因，这一数量大大高于基于遗传和生化研究的预期。不同基因中的特定插入已重组到染色体中，并对突变进行了表型特征分析。互补分析表明，这些基因排列在三个以相反方向转录的不同操纵子中。基于互补分析、融合蛋白数据和表型特征构建了该区域的详细物理图谱。此外，通过与已知的鼠伤寒沙门氏菌LPS突变体进行互补分析，确定了一些基因在LPS合成中的作用。该位点的遗传组织在大肠杆菌K-12和鼠伤寒沙门氏菌中似乎是相同的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/205993/0531f30829f9/jbacter00076-0169-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/205993/0adf4fa66ce1/jbacter00076-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/205993/0531f30829f9/jbacter00076-0169-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/205993/0adf4fa66ce1/jbacter00076-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/205993/0531f30829f9/jbacter00076-0169-a.jpg

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