转座子Tn10为相邻序列的转录提供启动子。
Transposon Tn10 provides a promoter for transcription of adjacent sequences.
作者信息
Ciampi M S, Schmid M B, Roth J R
出版信息
Proc Natl Acad Sci U S A. 1982 Aug;79(16):5016-20. doi: 10.1073/pnas.79.16.5016.
Abstract
Promoters located within the Tn10 insertion element cause transcription of "host" sequences adjacent to both ends of the inserted Tn10 element. These promoters are usually not observed in genetic experiments because their transcripts are efficiently terminated at nearby rho-dependent termination sites. The observations presented here provide an explanation for several confusing aspects of transposon behavior and suggest the possibility that many transposons possess promoters that have escaped detection for similar reasons.
摘要
位于Tn10插入元件内的启动子会导致与插入的Tn10元件两端相邻的“宿主”序列发生转录。在遗传实验中通常观察不到这些启动子，因为它们的转录本会在附近的ρ依赖性终止位点高效终止。本文所呈现的观察结果为转座子行为的几个令人困惑的方面提供了解释，并暗示了许多转座子可能拥有因类似原因而未被检测到的启动子的可能性。
相似文献
1
Transposon Tn10 provides a promoter for transcription of adjacent sequences.转座子Tn10为相邻序列的转录提供启动子。
Proc Natl Acad Sci U S A. 1982 Aug;79(16):5016-20. doi: 10.1073/pnas.79.16.5016.
2
Activation of silent genes by transposons Tn5 and Tn10.转座子Tn5和Tn10对沉默基因的激活作用。
Genetics. 1988 Dec;120(4):875-85. doi: 10.1093/genetics/120.4.875.
3
Transcriptional occlusion of transposon targets.转座子靶标的转录阻碍
Mol Gen Genet. 1989 Apr;216(2-3):204-9. doi: 10.1007/BF00334357.
4
Transcriptional activity of the transposable element Tn10 in the Salmonella typhimurium ilvGEDA operon.鼠伤寒沙门氏菌ilvGEDA操纵子中转座子Tn10的转录活性。
Proc Natl Acad Sci U S A. 1982 Aug;79(16):5011-5. doi: 10.1073/pnas.79.16.5011.
5
A consensus motif common to all Rho-dependent prokaryotic transcription terminators.所有依赖Rho的原核转录终止子共有的一致基序。
Cell. 1991 Feb 8;64(3):553-63. doi: 10.1016/0092-8674(91)90239-u.
6
Isolation of F' plasmids carrying a portion of the Salmonella typhimurium histidine transport operon.携带鼠伤寒沙门氏菌组氨酸转运操纵子一部分的F'质粒的分离。
J Bacteriol. 1984 Mar;157(3):697-702. doi: 10.1128/jb.157.3.697-702.1984.
7
Evidence for a common mechanism for the insertion of the Tn10 transposon and for the generation of Tn10-stimulated deletions.
Mol Gen Genet. 1978 Oct 30;166(2):217-23. doi: 10.1007/BF00285924.
8
Transposon Tn10: genetic organization, regulation, and insertion specificity.转座子Tn10：遗传组织、调控及插入特异性
Fed Proc. 1982 Aug;41(10):2649-52.
9
Convergently functional, Rho-independent terminator in Salmonella typhimurium.鼠伤寒沙门氏菌中具有收敛功能的、不依赖 Rho 因子的终止子。
J Bacteriol. 1985 Jul;163(1):362-8. doi: 10.1128/jb.163.1.362-368.1985.
10
Internal promoters of the his operon in Salmonella typhimurium.鼠伤寒沙门氏菌中组氨酸操纵子的内部启动子。
J Bacteriol. 1983 Feb;153(2):1114-9. doi: 10.1128/jb.153.2.1114-1119.1983.
引用本文的文献
1
Pasteurella multocida filamentous hemagglutinin B1 (fhaB1) gene is not involved with avian fowl cholera pathogenesis in turkey poults.多杀性巴氏杆菌丝状血凝素B1（fhaB1）基因与火鸡雏鸡禽霍乱发病机制无关。
BMC Vet Res. 2025 Mar 26;21(1):207. doi: 10.1186/s12917-025-04668-1.
2
A growth-coupling strategy for improving the stability of terpenoid bioproduction in Escherichia coli.一种提高萜类化合物生物生产在大肠杆菌中稳定性的生长偶联策略。
Microb Cell Fact. 2024 Oct 16;23(1):279. doi: 10.1186/s12934-024-02548-1.
3
Differential Afa/Dr Fimbriae Expression in the Multidrug-Resistant Escherichia coli ST131 Clone.多药耐药型大肠杆菌 ST131 克隆中 Afa/Dr 菌毛的差异表达。
mBio. 2022 Feb 22;13(1):e0351921. doi: 10.1128/mbio.03519-21. Epub 2022 Jan 18.
4
Upregulation of a homolog by transposon insertion promotes leafy head development in lettuce.转座子插入促进同源物上调，从而促进生菜的多叶头发育。
Proc Natl Acad Sci U S A. 2020 Dec 29;117(52):33668-33678. doi: 10.1073/pnas.2019698117. Epub 2020 Dec 7.
5
The N-terminus of IFT46 mediates intraflagellar transport of outer arm dynein and its cargo-adaptor ODA16.IFT46的N端介导外臂动力蛋白及其货物适配器ODA16的鞭毛内运输。
Mol Biol Cell. 2017 Sep 1;28(18):2420-2433. doi: 10.1091/mbc.E17-03-0172. Epub 2017 Jul 12.
6
A Natural Vibrio parahaemolyticus Δ Mutant Kills Shrimp but Produces neither Pir Toxins nor Acute Hepatopancreatic Necrosis Disease Lesions.一株天然副溶血性弧菌Δ突变体可杀死虾类，但既不产生Pir毒素，也不形成急性肝胰腺坏死病病变。
Appl Environ Microbiol. 2017 Aug 1;83(16). doi: 10.1128/AEM.00680-17. Print 2017 Aug 15.
7
Zinc-Induced Transposition of Insertion Sequence Elements Contributes to Increased Adaptability of Cupriavidus metallidurans.锌诱导插入序列元件的转位有助于提高金属抗性贪铜菌的适应性。
Front Microbiol. 2016 Mar 23;7:359. doi: 10.3389/fmicb.2016.00359. eCollection 2016.
8
Bacterial genome instability.细菌基因组不稳定性。
Microbiol Mol Biol Rev. 2014 Mar;78(1):1-39. doi: 10.1128/MMBR.00035-13.
9
A bacterial genome in transition--an exceptional enrichment of IS elements but lack of evidence for recent transposition in the symbiont Amoebophilus asiaticus.一个处于转变中的细菌基因组——共生菌亚洲拟无枝菌酸菌中 IS 元件的异常富集，但没有最近转座的证据。
BMC Evol Biol. 2011 Sep 26;11:270. doi: 10.1186/1471-2148-11-270.
10
Analysis of insertion sequences in thermophilic cyanobacteria: exploring the mechanisms of establishing, maintaining, and withstanding high insertion sequence abundance.嗜热蓝藻插入序列分析：探索建立、维持和承受高插入序列丰度的机制。
Appl Environ Microbiol. 2011 Aug;77(15):5458-66. doi: 10.1128/AEM.05090-11. Epub 2011 Jun 10.
本文引用的文献
1
Controlling elements and the gene.控制元件与基因
Cold Spring Harb Symp Quant Biol. 1956;21:197-216. doi: 10.1101/sqb.1956.021.01.017.
2
Genetic analysis of the histidine operon control region of Salmonella typhimurium.鼠伤寒沙门氏菌组氨酸操纵子控制区的遗传分析。
J Mol Biol. 1981 Feb 5;145(4):713-34. doi: 10.1016/0022-2836(81)90311-9.
3
Genetic analysis of a temperature-sensitive Salmonella typhimurium rho mutant with an altered rho-associated polycytidylate-dependent adenosine triphosphatase activity.对一株温度敏感型鼠伤寒沙门氏菌rho突变体的遗传分析，该突变体具有改变的rho相关聚胞苷酸依赖性三磷酸腺苷酶活性。
J Bacteriol. 1981 Jul;147(1):13-24. doi: 10.1128/jb.147.1.13-24.1981.
4
Transcriptional activity of the transposable element Tn10 in the Salmonella typhimurium ilvGEDA operon.鼠伤寒沙门氏菌ilvGEDA操纵子中转座子Tn10的转录活性。
Proc Natl Acad Sci U S A. 1982 Aug;79(16):5011-5. doi: 10.1073/pnas.79.16.5011.
5
Insertion of DNA activates the cryptic bgl operon in E. coli K12.DNA的插入激活了大肠杆菌K12中隐秘的bgl操纵子。
Nature. 1981 Oct 22;293(5834):625-9. doi: 10.1038/293625a0.
6
Genetic fusions that place the lactose genes under histidine operon control.
J Mol Biol. 1981 Feb 5;145(4):697-712. doi: 10.1016/0022-2836(81)90310-7.
7
Transposable elements associated with constitutive expression of yeast alcohol dehydrogenase II.与酵母乙醇脱氢酶II组成型表达相关的转座元件
Cell. 1981 Feb;23(2):605-14. doi: 10.1016/0092-8674(81)90156-2.
8
Mating signals control expression of mutations resulting from insertion of a transposable repetitive element adjacent to diverse yeast genes.交配信号控制着因一个可转座重复元件插入到多种酵母基因附近而产生的突变的表达。
Cell. 1980 Nov;22(2 Pt 2):427-36. doi: 10.1016/0092-8674(80)90353-0.
9
DNA rearrangements associated with a transposable element in yeast.酵母中与转座元件相关的DNA重排
Cell. 1980 Aug;21(1):239-49. doi: 10.1016/0092-8674(80)90131-2.
10
Polarity of Tn5 insertion mutations in Escherichia coli.大肠杆菌中Tn5插入突变的极性
J Bacteriol. 1980 May;142(2):439-46. doi: 10.1128/jb.142.2.439-446.1980.
Zotero 插件