Harvey Steven P, Minter Jennifer M
US Army Edgewood Chemical Biological Center, 5138 Blackhawk Road, Aberdeen Proving Ground, MD 21010-5424, USA.
FEMS Immunol Med Microbiol. 2005 Apr 1;44(1):91-7. doi: 10.1016/j.femsim.2004.12.002.
In this study, the subspecies differentiation of 25 isolates of Burkholderia mallei was attempted based on their ribotype polymorphisms. The isolates were from human and equine infections that occurred at various times around the world. DNA samples from each isolate were digested separately with PstI and EcoRI enzymes and probed with an Escherichia coli-derived 18-mer rDNA sequence to identify diagnostic fragments. Seventeen distinct ribotypes were identified from the combined data obtained with the two restriction enzymes. The results demonstrate the general utility of ribotyping for the subspecies identification of B. mallei isolates.
在本研究中，基于25株鼻疽伯克霍尔德菌分离株的核糖体分型多态性对其亚种分化进行了尝试。这些分离株来自世界各地不同时期发生的人类和马的感染病例。将每个分离株的DNA样本分别用PstI和EcoRI酶进行消化，并用源自大肠杆菌的18聚体rDNA序列进行探针杂交以鉴定诊断性片段。从两种限制性酶获得的合并数据中鉴定出17种不同的核糖体分型。结果证明了核糖体分型在鼻疽伯克霍尔德菌分离株亚种鉴定中的普遍实用性。
