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脉冲场凝胶电泳作为生物威胁因子鼻疽伯克霍尔德菌的鉴别分型技术。

Pulsed-field gel electrophoresis as a discriminatory typing technique for the biothreat agent burkholderia mallei.

作者信息

Chantratita Narisara, Vesaratchavest Mongkol, Wuthiekanun Vanaporn, Tiyawisutsri Rachaneeporn, Ulziitogtokh Tsedev, Akcay Erhan, Day Nicholas P J, Peacock Sharon J

机构信息

Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

出版信息

Am J Trop Med Hyg. 2006 Mar;74(3):345-7.

PMID:16525089
Abstract

Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) was used to type 21 laboratory strains of Burkholderia mallei. We demonstrated good resolution by PFGE together with clustering of some geographically related isolates, and confirmed previous observations that B. mallei is clonal as defined by MLST.

摘要

采用脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）对21株鼻疽伯克霍尔德菌实验室菌株进行分型。我们通过PFGE展示了良好的分辨能力以及一些地理相关分离株的聚类情况，并证实了之前的观察结果，即按照MLST的定义，鼻疽伯克霍尔德菌是克隆性的。

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