Chung Jing Yeng, Wilkie Ian, Boyce John D, Adler Ben
Australian Research Council Centre for Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Vic. 3800, Australia.
Vaccine. 2005 Apr 15;23(21):2751-5. doi: 10.1016/j.vaccine.2004.11.036.
We have previously constructed an acapsular Pasteurella multocida X-73 (serogroup A) mutant strain which was attenuated in virulence for chickens (Chung JY, Wilkie IW, Boyce JD, Townsend KM, Frost AJ, Ghodussi M, Adler B. Role of capsule in the pathogenesis of fowl cholera caused by Pasteurella multocida serogroup A. Infect. Immun. 2001;69:2487-2492). In this study, we have assessed the ability of this acapsular strain (PBA930) to induce protection against wild-type challenge in mice and the natural host chickens. Intramuscular administration of PBA930 to mice stimulated significant protection against X-73 and the heterologous strain P-1059 (A:3), but not against challenge with P-1662 (A:4). No protection was observed when PBA930 was introduced by the intraperitoneal or subcutaneous routes in mice. Significantly, the acapsular strain PBA930 was able to induce protection against challenge with wild type X-73 in chickens.
我们之前构建了一株无荚膜多杀性巴氏杆菌X-73（A血清群）突变株，该突变株对鸡的毒力减弱（Chung JY, Wilkie IW, Boyce JD, Townsend KM, Frost AJ, Ghodussi M, Adler B. 荚膜在A血清群多杀性巴氏杆菌引起的禽霍乱发病机制中的作用。感染与免疫。2001年；69:2487 - 2492）。在本研究中，我们评估了这株无荚膜菌株（PBA930）在小鼠和天然宿主鸡中诱导针对野生型攻击的保护能力。给小鼠肌肉注射PBA930可刺激产生针对X-73和异源菌株P-1059（A:3）的显著保护作用，但对P-1662（A:4）的攻击无保护作用。当通过腹腔或皮下途径给小鼠接种PBA930时，未观察到保护作用。值得注意的是，无荚膜菌株PBA930能够诱导鸡产生针对野生型X-73攻击的保护作用。
