Dick J W, Avakian A P
Department of Poultry Science, Clemson University, South Carolina 29634-0379.
Avian Dis. 1991 Oct-Dec;35(4):761-6.
Broiler chickens, in groups of 10, received a single vaccination by the stick-wing route at 1, 2, 3, 4, 5, 6, or 11 weeks of age with live Clemson University strain of Pasteurella multocida. Twenty non-vaccinates kept in isolation served as controls. Cholera serum antibody titers in all chickens were determined by enzyme-linked immunosorbent assay at weekly intervals. Chickens vaccinated once at 1, 2, 3, 4, 5, and 6 weeks, respectively, attained 25.2%, 28.7%, 34.7%, 46.2%, 51.8%, and 64.6% of the titers of those vaccinated once at 11 weeks of age. Variation in antibody response was greatest in chickens vaccinated at 1 or 2 weeks of age. Additionally, chickens vaccinated at 1 or 2 weeks of age showed the longest response time (5 weeks) to reach maximum antibody titers after a single vaccination. When the original vaccinates were revaccinated at 11 weeks of age, all showed a secondary response equal to or greater than that seen in chickens vaccinated once at 11 weeks of age. Age of the chickens at the time of vaccination and antibody titer were positively correlated (r = 0.997). Overall antibody responses to vaccination were higher and much more uniform as birds increased in age.
将10只一组的肉鸡在1、2、3、4、5、6或11周龄时通过翼下途径用克莱姆森大学多杀巴斯德氏菌活菌株进行单次疫苗接种。将20只单独饲养的未接种疫苗的鸡作为对照。每周通过酶联免疫吸附测定法测定所有鸡的霍乱血清抗体滴度。分别在1、2、3、4、5和6周龄时接种一次疫苗的鸡，其抗体滴度分别达到11周龄时接种一次疫苗的鸡的滴度的25.2%、28.7%、34.7%、46.2%、51.8%和64.6%。在1或2周龄时接种疫苗的鸡中，抗体反应的差异最大。此外，在1或2周龄时接种疫苗的鸡在单次接种后达到最大抗体滴度的反应时间最长（5周）。当原来接种过疫苗的鸡在11周龄时再次接种疫苗时，所有鸡都表现出与11周龄时单次接种疫苗的鸡相同或更高的二次反应。接种疫苗时鸡的年龄与抗体滴度呈正相关（r = 0.997）。随着鸡龄的增加，总体疫苗接种抗体反应更高且更均匀。
