Itoh Tomohito, Watanabe Toshio, Ihara Naoya, Mariani Paola, Beattie Craig W, Sugimoto Yoshikazu, Takasuga Akiko
Shirakawa Institute of Animal Genetics, Odakura, Nishigo, Nishi-shirakawa, Fukushima 961-8061, Japan.
Genomics. 2005 Apr;85(4):413-24. doi: 10.1016/j.ygeno.2004.12.007.
A bovine whole genome 7000-rad radiation hybrid (RH) panel, SUNbRH(7000-rad), was constructed to build a high-resolution RH map. The Shirakawa-USDA linkage map served as a scaffold to construct a framework map of 3216 microsatellites on which 2377 ESTs were ordered. The resulting RH map provided essentially complete coverage across the genome, with 1 cR7000 corresponding to 114 kb, and a cattle-human comparative map of 1716 bovine genes and sequences annotated in the human genome, which covered 79 and 72% of the bovine and human genomes, respectively. We then integrated the bovine RH and comparative maps with BAC fingerprint information in to construct a detailed, BAC-based physical map covering a reported 40-cM quantitative trait locus region for intramuscular fat or "marbling" on BTA 4. In summary, the new, high-resolution SUNbRH7000-rad, comparative, Shirakawa-USDA linkage, and BAC fingerprint maps provide a set of genomic tools for fine mapping regions of interest in cattle.
构建了一个牛全基因组7000拉德辐射杂种（RH）面板SUNbRH(7000-rad)，以构建高分辨率的RH图谱。白川-美国农业部连锁图谱作为构建3216个微卫星框架图谱的支架，在该框架图谱上排列了2377个EST。所得的RH图谱基本覆盖了整个基因组，1 cR7000相当于114 kb，还有一张牛-人比较图谱，包含1716个在人类基因组中注释的牛基因和序列，分别覆盖了牛和人类基因组的79%和72%。然后，我们将牛RH图谱和比较图谱与BAC指纹信息整合，构建了一张详细的基于BAC的物理图谱，该图谱覆盖了报道的位于BTA 4上的肌内脂肪或“大理石花纹”的40-cM数量性状基因座区域。总之，新的高分辨率SUNbRH7000-rad、比较图谱、白川-美国农业部连锁图谱和BAC指纹图谱为精细定位牛中感兴趣的区域提供了一套基因组工具。
