Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA.
Genome Res. 2010 Aug;20(8):1122-32. doi: 10.1101/gr.104216.109. Epub 2010 May 27.
Using radiation hybrid genotyping data, 99% of all possible gene pairs across the mammalian genome were tested for interactions based on co-retention frequencies higher (attraction) or lower (repulsion) than chance. Gene interaction networks constructed from six independent data sets overlapped strongly. Combining the data sets resulted in a network of more than seven million interactions, almost all attractive. This network overlapped with protein-protein interaction networks on multiple measures and also confirmed the relationship between essentiality and centrality. In contrast to other biological networks, the radiation hybrid network did not show a scale-free distribution of connectivity but was Gaussian-like, suggesting a closer approach to saturation. The radiation hybrid (RH) network constitutes a platform for understanding the systems biology of the mammalian cell.
利用辐射杂种基因分型数据，基于高于（吸引）或低于（排斥）偶然的共保留频率，对哺乳动物基因组中所有可能的基因对进行了 99%的相互作用测试。由六个独立数据集构建的基因相互作用网络强烈重叠。将数据集结合起来，得到了一个包含超过 700 万个相互作用的网络，几乎都是有吸引力的。该网络在多个指标上与蛋白质-蛋白质相互作用网络重叠，也证实了必需性和中心性之间的关系。与其他生物网络不同，辐射杂种网络没有表现出无标度连接性的分布，而是类高斯分布，这表明更接近饱和。辐射杂种（RH）网络为理解哺乳动物细胞的系统生物学提供了一个平台。
