de Oliveira Poersch Celina, Pavoni Daniela Parada, Queiroz Mario H, de Borba Luana, Goldenberg Samuel, dos Santos Claudia Nunes Duarte, Krieger Marco Aurélio
Instituto de Biologia Molecular do Paraná, IBMP, Rua Prof. Algacyr Munhoz Mader, 3775, 81350-010 Curitiba, Paraná, Brazil.
J Clin Virol. 2005 Apr;32(4):272-7. doi: 10.1016/j.jcv.2004.08.008.
The control of dengue depends solely on the control of the insect vector and efficient diagnosis of human cases as no vaccines or specific treatments are currently available. Existing diagnostic methods for suspected clinical cases are complicated by the short duration of viraemia and by serological cross-reactivity with epitopes from other flaviviruses.
To evaluate PCR-based tests (nested reverse transcription (RT)-PCR and real-time RT-PCR) for the detection and serotyping of dengue virus and compare the results with those obtained with a widely used immunological test (IgM antibody capture ELISA-MAC-ELISA).
The PCR-based methods were more effective in the first few days of infection, whereas the MAC-ELISA became more sensitive 5 or 6 days after disease onset. These results suggest that the best method for dengue diagnosis is a combination of PCR-based and immunological tests. Real-time RT-PCR was more sensitive than the nested RT-PCR approach. Furthermore, it was rapid, reproducible and highly specific, making it a potential method for the diagnosis of dengue fever.
由于目前尚无疫苗或特效治疗方法，登革热的防控完全依赖于病媒昆虫的控制以及对人类病例的有效诊断。现有针对疑似临床病例的诊断方法因病毒血症持续时间短以及与其他黄病毒表位的血清学交叉反应而变得复杂。
评估基于聚合酶链反应（PCR）的检测方法（巢式逆转录（RT）-PCR和实时RT-PCR）用于登革病毒的检测和血清分型，并将结果与广泛使用的免疫检测方法（IgM抗体捕获酶联免疫吸附测定-MAC-ELISA）所获结果进行比较。
基于PCR的方法在感染后的头几天更有效，而MAC-ELISA在发病5或6天后变得更敏感。这些结果表明，登革热诊断的最佳方法是将基于PCR的检测方法与免疫检测方法相结合。实时RT-PCR比巢式RT-PCR方法更敏感。此外，它快速、可重复且高度特异，使其成为诊断登革热的一种潜在方法。
