用于检测登革病毒的快速免疫层析NS1检测、抗登革热IgM检测、半巢式PCR及IgM ELISA的评估。
Evaluation of rapid immunochromatographic NS1 test, anti-dengue IgM test, semi-nested PCR and IgM ELISA for detection of dengue virus.
作者信息
Tontulawat Praewpilai, Pongsiri Piyathida, Thongmee Chittima, Theamboonlers Apiradee, Kamolvarin Nuantip, Poovorawan Yong
机构信息
Department of Biochemistry, Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
出版信息
Southeast Asian J Trop Med Public Health. 2011 May;42(3):570-8.
Dengue virus (DENV) causes various clinical symptoms of differing severity based on time of infections. The existing laboratory methods, semi-nested PCR and Dengue IgM ELISA, still have limitations for diagnosis. A commercially available rapid immunochromatographic dengue NS1 antigen and IgM antibody tests in comparison with semi-nested PCR and IgM ELISA for confirmation of DENV infection were evaluated. In total, 237 single acute serum specimens and 50 paired sera of dengue patients were examined using the rapid dengue NS1 antigen test, IgM antibody test, semi-nested PCR and Dengue IgM ELISA. The NS1 and IgM rapid tests showed sensitivity of 70.6%, and 75.6%, respectively, and specificity of 73.4% and 97.1%, respectively. The combination of NS1 and IgM tests enhanced diagnosis. Thus rapid dengue NS1 antigen and IgM antibody tests are highly appropriate for diagnosis of dengue infection as it is rapid, easily applicable, sensitive and highly specific.
登革病毒（DENV）根据感染时间会引发不同严重程度的各种临床症状。现有的实验室方法，即半巢式聚合酶链反应（PCR）和登革热免疫球蛋白M（IgM）酶联免疫吸附测定（ELISA），在诊断方面仍存在局限性。对一种市售的快速免疫层析法登革热非结构蛋白1（NS1）抗原和IgM抗体检测方法与半巢式PCR和IgM ELISA进行比较，以评估其对登革病毒感染的确诊能力。总共使用快速登革热NS1抗原检测、IgM抗体检测、半巢式PCR和登革热IgM ELISA对237份单一急性血清标本和50份登革热患者的配对血清进行了检测。NS1和IgM快速检测的灵敏度分别为70.6%和75.6%，特异性分别为73.4%和97.1%。NS1和IgM检测相结合提高了诊断效果。因此，快速登革热NS1抗原和IgM抗体检测非常适合用于登革热感染的诊断，因为它快速、易于操作、灵敏且高度特异。
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